Construct: ORF BRDN0000556260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- n/a
- Derived from:
- BRDN0000464765
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HcRed (HcRed)
Vector Information:
- Vector Backbone:
- pLXI_403
- Pol II Cassette 1:
- TRE-Gateway-V5
- Pol II Cassette 2:
- PGK-PuroR-2A-rtTA-VP16
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | HcRed | HcRed | Hahn Lab HcRed | HcRed.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 759
- ORF length:
- 684
- Sequence:
-
1 gctcgtttag tgaaccgtca gatcgcctgg agaattggct agcatcaaca agtttgtaca 61 aaaaagttgg caccatggtg agcggcctgc tgaaggagag tatgcgcatc aagatgtaca 121 tggagggcac cgtgaacggc cactacttca agtgcgaggg cgagggcgac ggcaacccct 181 tcgccggcac ccagagcatg agaatccacg tgaccgaggg cgcccccctg cccttcgcct 241 tcgacatcct ggccccctgc tgcgagtacg gcagcaggac cttcgtgcac cacaccgccg 301 agatccccga cttcttcaag cagagcttcc ccgagggctt cacctgggag agaaccacca 361 cctacgagga cggcggcatc ctgaccgccc accaggacac cagcctggag ggcaactgcc 421 tgatctacaa ggtgaaggtg cacggcacca acttccccgc cgacggcccc gtgatgaaga 481 acaagagcgg cggctgggag cccagcaccg aggtggtgta ccccgagaac ggcgtgctgt 541 gcggccggaa cgtgatggcc ctgaaggtgg gcgaccggca cctgatctgc caccactaca 601 ccagctaccg gagcaagaag gccgtgcgcg ccctgaccat gcccggcttc cacttcaccg 661 acatccggct ccagatgctg cggaagaaga aggacgagta cttcgagctg tacgaggcca 721 gcgtggcccg gtacagcgac ctgcccgaga aggccaactt gccaactttc ttgtacaaag 781 tggttgcagg aaagccaatc ccaaacccac tcctaggtct cgacagtaca gcatagaccg 841 gtccaccacc accaccacca ctaaggatcc ggggttgggg ttgcgc