Construct: ORF BRDN0000556266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- n/a
- Derived from:
- BRDN0000464771
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LacZ (LacZ)
Vector Information:
- Vector Backbone:
- pLXI_401
- Pol II Cassette 1:
- TRE-Gateway-No Tag
- Pol II Cassette 2:
- PGK-PuroR-2A-rtTA-VP16
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | LacZ | LacZ | Hahn Lab LacZ | LacZ.1 | 100% | 100% | |
| 2 | CONTROL | lacZ | lacZ | lacZ | lacZ.1 | 91.7% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3279
- ORF length:
- 3204
- Sequence:
-
1 gctcgtttag tgaaccgtca gatcgcctgg agaattggct agcatcaaca agtttgtaca 61 aaaaagttgg caccatgata gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg 121 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 181 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct 241 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg 301 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct 361 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga 421 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc 481 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg 541 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg 601 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc 661 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta 721 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac 781 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt 841 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg 901 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac 961 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg 1021 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg 1081 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc 1141 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga 1201 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca 1261 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca 1321 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg 1381 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga 1441 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc 1501 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt 1561 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca 1621 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc 1681 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc 1741 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg 1801 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc 1861 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag 1921 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca 1981 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg 2041 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga 2101 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg 2161 tagtgcaacc gaacgcgacc gcatggtcag aagccggcca catcagcgcc tggcagcagt 2221 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc 2281 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc 2341 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc 2401 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc 2461 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag 2521 cagcgttgtt gcagtgcacg gcagatacac ttgctgacgc ggtgctgatt acgaccgctc 2581 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg 2641 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg 2701 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat 2761 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc 2821 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg 2881 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca 2941 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag 3001 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggagac gactcctgga 3061 gcccgtcagt atcggcggaa ttacagctga gcgccggtcg ctaccattac cagttggtct 3121 ggtgtcaaaa aactaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata 3181 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc 3241 ctaaccctct cctcggtctc gattctacgc gtaccggttt gccaactttc ttgtacaaag 3301 tggtttagta atgaaccggt ccaccaccac caccaccact aaggatccgg ggttggggtt 3361 gcgc