Construct: ORF BRDN0000556266

Construct Description:

Construct Type:
ORF
Other Identifiers:
n/a
Derived from:
BRDN0000464771
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LacZ (LacZ)

Vector Information:

Vector Backbone:
pLXI_401
Pol II Cassette 1:
TRE-Gateway-No Tag
Pol II Cassette 2:
PGK-PuroR-2A-rtTA-VP16
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 CONTROL LacZ LacZ Hahn Lab LacZ LacZ.1 100% 100%
2 CONTROL lacZ lacZ lacZ lacZ.1 91.7% 92.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
3279
ORF length:
3204
Sequence:
1gctcgtttag tgaaccgtca gatcgcctgg agaattggct agcatcaaca agtttgtaca
61aaaaagttgg caccatgata gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
121gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
181aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
241ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
301aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
361acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
421cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
481gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
541gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
601gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
661aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
721cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
781tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
841tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
901atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
961tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
1021gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
1081gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
1141atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
1201agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
1261cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
1321tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
1381taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
1441atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
1501cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
1561gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
1621tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
1681acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
1741cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
1801aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
1861agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag
1921caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca
1981gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg
2041gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga
2101ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg
2161tagtgcaacc gaacgcgacc gcatggtcag aagccggcca catcagcgcc tggcagcagt
2221ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc
2281tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc
2341gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc
2401tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
2461gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
2521cagcgttgtt gcagtgcacg gcagatacac ttgctgacgc ggtgctgatt acgaccgctc
2581acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
2641gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg
2701cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat
2761tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc
2821tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
2881ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
2941gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
3001aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggagac gactcctgga
3061gcccgtcagt atcggcggaa ttacagctga gcgccggtcg ctaccattac cagttggtct
3121ggtgtcaaaa aactaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata
3181tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
3241ctaaccctct cctcggtctc gattctacgc gtaccggttt gccaactttc ttgtacaaag
3301tggtttagta atgaaccggt ccaccaccac caccaccact aaggatccgg ggttggggtt
3361gcgc

Download FASTA (ORF) (Full)