Construct: ORF BRDN0000556279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- n/a
- Derived from:
- BRDN0000464771
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LacZ (LacZ)
Vector Information:
- Vector Backbone:
- pLX_314
- Pol II Cassette 1:
- SV40-NeoR
- Pol II Cassette 2:
- EF1a-BRDN0000556279
- Selection Marker:
- NeoR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | LacZ | LacZ | Hahn Lab LacZ | LacZ.1 | 100% | 100% | |
| 2 | CONTROL | lacZ | lacZ | lacZ | lacZ.1 | 91.7% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3273
- ORF length:
- 3204
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 121 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 181 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct 241 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg 301 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca 361 acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt 421 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta 481 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg 541 gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa 601 accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata 661 tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa 721 tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg 781 ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc 841 agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc 901 gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga 961 gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc 1021 tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc 1081 tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc 1141 tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga 1201 acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt 1261 gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc 1321 caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc 1381 gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat 1441 caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc 1501 gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga 1561 tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa 1621 aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga 1681 tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt 1741 tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg 1801 gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct 1861 gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac 1921 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat 1981 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc 2041 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac 2101 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc 2161 aaccgaacgc gaccgcatgg tcagaagccg gccacatcag cgcctggcag cagtggcgtc 2221 tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca 2281 ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt 2341 caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg 2401 atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg 2461 accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt 2521 tgttgcagtg cacggcagat acacttgctg acgcggtgct gattacgacc gctcacgcgt 2581 ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg 2641 gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga 2701 ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc 2761 cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat 2821 tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc 2881 gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct 2941 acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca 3001 catggctgaa tatcgacggt ttccatatgg ggattggtgg agacgactcc tggagcccgt 3061 cagtatcggc ggaattacag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc 3121 aaaaaactaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt gatatccagc 3181 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc 3241 ctctcctcgg tctcgattct acgcgtaccg gtttgccaac tttcttgtac aaagtggttg 3301 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta 3361 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacct