Construct: ORF BRDN0000556289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- n/a
- Derived from:
- BRDN0000464768
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- Luciferase (Luciferase)
Vector Information:
- Vector Backbone:
- pLXI_403
- Pol II Cassette 1:
- TRE-Gateway-V5
- Pol II Cassette 2:
- PGK-PuroR-2A-rtTA-VP16
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | Luciferase | Luciferase | Hahn Lab Luciferase | Luciferase.1 | 100% | 100% | |
| 2 | CONTROL | LUCIFERASE | LUCIFERASE | promegaLuc | promegaLuc.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1725
- ORF length:
- 1650
- Sequence:
-
1 gctcgtttag tgaaccgtca gatcgcctgg agaattggct agcatcaaca agtttgtaca 61 aaaaagttgg caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc 121 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg 181 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt 241 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc 301 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt 361 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca 421 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa 481 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa 541 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt 601 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca 661 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct 721 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg 781 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt 841 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga 901 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg 961 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg 1021 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta 1081 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg 1141 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc 1201 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta 1261 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg 1321 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg 1381 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat 1441 ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg 1501 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa 1561 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag 1621 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca 1681 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgttgcca actttcttgt 1741 acaaagtggt tgcaggaaag ccaatcccaa acccactcct aggtctcgac agtacagcat 1801 agaccggtcc accaccacca ccaccactaa ggatccgggg ttggggttgc gc