Construct: sgRNA BRDN0000562805

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTGCCCATCCTGGTCGAGC

Original Target:

Taxon:
Control
Gene:
eGFP (eGFP)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80035

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 232485266 + ECEL1 NNNGCCCATCCTGGTCAATC NGG 2 0.6222 Tier I
2 NC_000005.10 5 138465754 - EGR1 NNNGGCCATCCTGGACGAGC NGG 2 0.3474 Tier I
3 NC_000011.10 11 118533032 - TMEM25 NNNGCCCATCCTGGTCGATC NAG 2 0.1728 Tier I
4 NC_000019.10 19 1119984 + SBNO2 NNNGCCCATCTTGGTGGAGC NGG 2 0.0473 Tier I
5 NC_000001.11 1 84843852 - LPAR3 NNNGCCCATCCAGGTAGAGC NGG 2 0.8 Tier II
6 NC_000006.12 6 79634950 + SH3BGRL2 NNNGCCCATCCTGCTCAAGC NGG 2 0.4 Tier II
7 NC_000015.10 15 72197167 - GRAMD2A NNNGCCCATCCCGGTCGGGC NGG 2 0.3789 Tier II
8 NC_000024.10 Y 13237437 - UTY NNNGCCCATCCTGGCCAAGC NGG 2 0.2545 Tier II
9 NC_000012.12 12 104736436 + CHST11 NNNGCCCAGCCTGGCCGAGC NGG 2 0.1688 Tier II
10 NC_000010.11 10 132915044 - CFAP46 NNNGCCCAGCCTGGTCTAGC NGG 2 0.1548 Tier II
11 NC_000012.12 12 47799804 - HDAC7 NNNGCCCAGCCTGGTCCAGC NGG 2 0.1457 Tier II
12 NC_000002.12 2 88078020 - SMYD1 NNNGCCCATCCTGGTCGGGC NTG 2 0.0156 Tier II
13 NC_000010.11 10 91601124 + HECTD2-AS1 NNNGTCCATCCTGGTCAAGC NGG 2 0.5939 Tier III
14 NC_000004.12 4 19442719 - LINC02438 NNNGCCAATCCTGGCCGAGC NGG 2 0.2045 Tier III
15 NC_000007.14 7 149825825 - SSPOP NNNGCCCATCCTGGACCAGC NGG 2 0.1362 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 87153585 + Ecel1 NNNGCCCATCCTGGTCAATC NGG 2 0.6222 Tier I
2 NC_000086.7 X 13486129 + Nyx NNNGCGCATCCTGGGCGAGC NGG 2 0.025 Tier I
3 NC_000071.6 5 48971549 + Kcnip4 NNNGGCCATCCTGGTCTAGC NGG 2 0.15 Tier II
4 NC_000081.6 15 84444988 - Shisal1 NNNGCCCATCCTGGTAGAGG NGG 2 0.0588 Tier II
5 NC_000070.6 4 103851977 - Dab1 NNNGCCCATCCTGGGAGAGC NGG 2 0.05 Tier II
6 NC_000075.6 9 98296680 + Nmnat3 NNNACCCATCCTGGTCGAGC NGC 2 0.02 Tier II
7 NC_000067.6 1 87153585 + Gm33292 NNNGCCCATCCTGGTCAATC NGG 2 0.6222 Tier III
Download CSV

Other clones with same target sequence:

(none)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.