Construct: sgRNA BRDN0001144720

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGACAAGAAAGCCAAACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK11 (79858)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75560

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 131029864 + NEK11 NNNGACAAGAAAGCCAAACG NGG 0 1.0 Tier I
2 NC_000018.10 18 52623959 + DCC NNNGACAAGAAAGCCAAACA NGG 1 0.9375 Tier II
3 NC_000007.14 7 86750806 + GRM3 NNNGAAAAGAAAGCCAAACT NGG 2 0.65 Tier II
4 NC_000019.10 19 38951954 - FBXO17 NNNGAAAAGAAAGCCAAACT NGG 2 0.65 Tier II
5 NC_000001.11 1 179644791 - TDRD5 NNNAACAAGAAAGCCAAACT NGG 2 0.63 Tier II
6 NC_000012.12 12 10603376 - MAGOHB NNNGACAAGAAAGACAAACA NGG 2 0.3281 Tier II
7 NC_000004.12 4 74984051 - PARM1 NNNGCCAAGAAAGCCAAATG NGG 2 0.2308 Tier II
8 NC_000011.10 11 86671612 + ME3 NNNGACAAGAAAGCCAAACC NAG 2 0.1111 Tier II
9 NC_000011.10 11 115216807 - CADM1 NNNGACCAGAAAGCCAAAGG NGG 2 0.0547 Tier II
10 NC_000002.12 2 190596161 + NEMP2 NNNGACAAGAAAACCAAACG NTG 2 0.036 Tier II
11 NC_000002.12 2 148105645 - MBD5 NNNGACAAGAAAGCTAAAAG NGG 2 0.0286 Tier II
12 NC_000023.11 X 11252694 + ARHGAP6 NNNGACAAGAAAGCCAAAGG NGC 2 0.0028 Tier II
13 NC_000011.10 11 112303552 + LINC02762 NNNGACATGAATGCCAAACG NGG 2 0.1429 Tier III
14 NC_000017.11 17 16980448 + LOC284191 NNNGACAAGAAGGCCAGACG NGG 2 0.1275 Tier III
15 NC_000002.12 2 190596161 + NEMP2-DT NNNGACAAGAAAACCAAACG NTG 2 0.036 Tier III
16 NC_000007.14 7 142627826 - TRB NNNGACAAGAAAGCCAATCG NTG 2 0.0195 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 105392864 - Nek11 NNNGACAAGAAAGCCAAACC NGG 1 0.4286 Tier I
2 NC_000081.6 15 92339561 - Cntn1 NNNGACAAGAAAGCCAAGGG NGG 2 0.05 Tier I
3 NC_000068.7 2 76318153 - Pde11a NNNGACAAGAAAACCAAAAG NGG 2 0.3956 Tier II
4 NC_000072.6 6 39055358 + Tbxas1 NNNAACAAGAAAGCCAAAAG NGG 2 0.3857 Tier II
5 NC_000080.6 14 117450189 + Gpc6 NNNGACAGGAAAGCCAAATG NGG 2 0.3385 Tier II
6 NC_000071.6 5 100364795 - Sec31a NNNGACAAGAAAGCCAAGCT NGG 2 0.28 Tier II
7 NC_000077.6 11 105969277 + Ace NNNGAGTAGAAAGCCAAACG NGG 2 0.2188 Tier II
8 NC_000071.6 5 108196446 - Ccdc18 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier II
9 NC_000078.6 12 17936997 - Gm9222 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier II
10 NC_000078.6 12 18981626 - Gm40847 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier II
11 NC_000078.6 12 20668085 - LOC108167368 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier II
12 NC_000078.6 12 23494809 + Gm40862 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier II
13 NC_000078.6 12 24304678 + Gm4120 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier II
14 NC_000072.6 6 54486177 - Scrn1 NNNGACAAGAAAGCCAAGAG NGG 2 0.1714 Tier II
15 NC_000072.6 6 54486177 - Wipf3 NNNGACAAGAAAGCCAAGAG NGG 2 0.1714 Tier II
16 NC_000078.6 12 103218182 + Prima1 NNNGACAAGAAAGCCAGACT NGG 2 0.1235 Tier II
17 NC_000080.6 14 21316489 - Adk NNNGGCAAGAAAGCCAAACG NGA 2 0.05 Tier II
18 NC_000076.6 10 69630816 - Ank3 NNNGACAAGAAAGCCAAAAG NGA 2 0.0298 Tier II
19 NC_000069.6 3 87445229 + Fcrl5 NNNGACAAGAAAGCCAAAAG NTG 2 0.0167 Tier II
20 NC_000076.6 10 76434183 + Pcnt NNNGACAAGACAGCCAAACG NTG 2 0.0156 Tier II
21 NC_000069.6 3 80689064 + Gria2 NNNGACAAGAAAGCTAAACG NGC 2 0.0015 Tier II
22 NC_000070.6 4 3741777 + Lyn NNNGACAAGAAAGCCTAACT NGG 2 0.0 Tier II
23 NC_000072.6 6 34365344 - Akr1b8 NNNGAGAAGAAAGCCTAACG NGG 2 0.0 Tier II
24 NC_000077.6 11 105969277 + Gm11651 NNNGAGTAGAAAGCCAAACG NGG 2 0.2188 Tier III
25 NC_000078.6 12 21626872 + Gm4036 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier III
26 NC_000078.6 12 22205031 + Gm9289 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier III
27 NC_000078.6 12 22956636 + 2410018L13Rik NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier III
28 NC_000078.6 12 23363791 + Gm9285 NNNGAGAAGAAAGCCAAACC NGG 2 0.2143 Tier III
29 NC_000067.6 1 184542675 + 1700112H15Rik NNNGATAAGAAAGCCAAACG NTG 2 0.0362 Tier III
30 NC_000086.7 X 153049901 - 9530051G07Rik NNNGACAAGAAAGCAAAAGG NGG 2 0.0278 Tier III
31 NC_000070.6 4 3513453 - LOC108168924 NNNGACAAGAAAGCCTAACT NGG 2 0.0 Tier III
32 NC_000076.6 10 111598519 + Gm30262 NNNGACAAGAAAGGCAACCG NGG 2 0.0 Tier III
33 NC_000072.6 6 34365344 - Gm13858 NNNGAGAAGAAAGCCTAACG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)