Construct: sgRNA BRDN0001144722
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTATGCACTGTCAATACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NRBP1 (29959)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77019
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
27433697 |
- |
NRBP1 |
NNNTATGCACTGTCAATACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
175701870 |
+ |
GPM6A |
NNNTTTGCACTGACAATACC |
NGG |
2 |
0.2517 |
Tier II |
| 3 |
NC_000013.11 |
13 |
92488678 |
- |
GPC5 |
NNNTATGCACTCTAAATACC |
NGG |
2 |
0.1853 |
Tier II |
| 4 |
NC_000001.11 |
1 |
235635020 |
- |
GNG4 |
NNNTATTCACTGTCTATACC |
NGG |
2 |
0.1143 |
Tier II |
| 5 |
NC_000001.11 |
1 |
33930372 |
+ |
CSMD2 |
NNNTATGCACTGTCCATACC |
NGA |
2 |
0.0189 |
Tier II |
| 6 |
NC_000013.11 |
13 |
32837822 |
+ |
LINC00423 |
NNNTTTGCACTGTCAAAACC |
NGG |
2 |
0.1939 |
Tier III |
| 7 |
NC_000013.11 |
13 |
92488678 |
- |
GPC5-IT1 |
NNNTATGCACTCTAAATACC |
NGG |
2 |
0.1853 |
Tier III |
| 8 |
NC_000004.12 |
4 |
25040406 |
- |
LOC124900684 |
NNNTTTGCACTGTCAATACC |
NGC |
2 |
0.0081 |
Tier III |
| 9 |
NC_000002.12 |
2 |
66005051 |
+ |
LINC02934 |
NNNTATGCACTGTGAATGCC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
31244190 |
- |
Nrbp1 |
NNNTATGCACTGTCAATGCC |
NGG |
1 |
0.4 |
Tier I |
| 2 |
NC_000077.6 |
11 |
86281912 |
- |
Med13 |
NNNTGTACACTGTCAATACC |
NGG |
2 |
0.72 |
Tier II |
| 3 |
NC_000086.7 |
X |
160077000 |
- |
Map3k15 |
NNNTAAGCACTGTCAATACC |
NAG |
2 |
0.2247 |
Tier II |
| 4 |
NC_000082.6 |
16 |
32863673 |
+ |
Rubcn |
NNNGATGCACTGTCAATCCC |
NGG |
2 |
0.119 |
Tier II |
| 5 |
NC_000079.6 |
13 |
112876147 |
+ |
Mtrex |
NNNTATGCACTGTAAATACT |
NGG |
2 |
0.105 |
Tier II |
| 6 |
NC_000072.6 |
6 |
137628237 |
- |
Eps8 |
NNNCATGCACTGTCATTACC |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000080.6 |
14 |
23645075 |
+ |
Kcnma1 |
NNNTAAGCACTGTCACTACC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000072.6 |
6 |
35268240 |
- |
Slc13a4 |
NNNTATGCACTGTCATTAAC |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000085.6 |
19 |
17726810 |
+ |
Pcsk5 |
NNNTATGCACTGTCATTACT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000085.6 |
19 |
45482872 |
+ |
Btrc |
NNNTATGCCCTGTCACTACC |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000077.6 |
11 |
86281912 |
- |
Brip1os |
NNNTGTACACTGTCAATACC |
NGG |
2 |
0.72 |
Tier III |
| 12 |
NC_000079.6 |
13 |
70725302 |
- |
Gm36607 |
NNNTATGCAATGTCAAAACC |
NGG |
2 |
0.4622 |
Tier III |
| 13 |
NC_000072.6 |
6 |
68765040 |
+ |
Igk |
NNNTATGTACTGTCAATACA |
NGG |
2 |
0.4375 |
Tier III |
| 14 |
NC_000081.6 |
15 |
5389295 |
- |
Gm46496 |
NNNTATGCAGTGTCTATACC |
NGG |
2 |
0.0778 |
Tier III |
| 15 |
NC_000080.6 |
14 |
23645075 |
+ |
Gm51422 |
NNNTAAGCACTGTCACTACC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
