Construct: sgRNA BRDN0001144728
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGATTCACCCTGTTACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K2 (5871)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76779
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64796814 |
- |
MAP4K2 |
NNNATTCACCCTGTTACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
45403670 |
+ |
SLC14A2 |
NNNATTCACCCTGTAACTCT |
NGG |
2 |
0.4053 |
Tier II |
| 3 |
NC_000016.10 |
16 |
50258949 |
+ |
ADCY7 |
NNNATTCACCCTGTTACAAG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000004.12 |
4 |
5935311 |
- |
C4orf50 |
NNNATTCCCCCTGTTACTGG |
NGG |
2 |
0.0536 |
Tier II |
| 5 |
NC_000003.12 |
3 |
109015242 |
- |
MORC1 |
NNNCTTCACCCTGTTACTGG |
NGG |
2 |
0.0441 |
Tier II |
| 6 |
NC_000004.12 |
4 |
175886178 |
- |
GPM6A |
NNNATTCACCCTGTTACTGG |
NAG |
2 |
0.0324 |
Tier II |
| 7 |
NC_000018.10 |
18 |
45403670 |
+ |
SLC14A2-AS1 |
NNNATTCACCCTGTAACTCT |
NGG |
2 |
0.4053 |
Tier III |
| 8 |
NC_000006.12 |
6 |
157707199 |
+ |
LOC107986663 |
NNNACTCACCCTGTTACTGG |
NGG |
2 |
0.125 |
Tier III |
| 9 |
NC_000011.10 |
11 |
11173177 |
- |
LINC02752 |
NNNATTCTCCCTGTTACTCG |
NAG |
2 |
0.1111 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6346289 |
+ |
Map4k2 |
NNNGTTCACCCTGTGACTCG |
NGG |
2 |
0.0313 |
Tier I |
| 2 |
NC_000068.7 |
2 |
76502541 |
- |
Osbpl6 |
NNNATTCACCCTGTTACTTG |
NTG |
2 |
0.018 |
Tier II |
| 3 |
NC_000079.6 |
13 |
45540817 |
+ |
Gmpr |
NNNATTCACCCTGTTACTCG |
NCA |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
