Construct: sgRNA BRDN0001144729
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCGCAAAGCAGGTGAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TKFC (26007)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75783
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
61341494 |
+ |
TKFC |
NNNCGCAAAGCAGGTGAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
162163868 |
- |
PRKN |
NNNTGCAGAGCAGGTGAACG |
NGG |
2 |
0.5867 |
Tier II |
| 3 |
NC_000019.10 |
19 |
5337344 |
+ |
PTPRS |
NNNCAGAAAGCAGGTGAACG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000016.10 |
16 |
77363459 |
- |
ADAMTS18 |
NNNCGCAAAGCAGGTGAATT |
NGG |
2 |
0.3231 |
Tier II |
| 5 |
NC_000003.12 |
3 |
122998514 |
+ |
SEMA5B |
NNNGGCAAAGCAGGTGAACG |
NGT |
2 |
0.0081 |
Tier II |
| 6 |
NC_000022.11 |
22 |
33875643 |
+ |
LARGE1 |
NNNCGCAAAGCAGGTGACCG |
NTG |
2 |
0.0074 |
Tier II |
| 7 |
NC_000019.10 |
19 |
5337344 |
+ |
LOC105372252 |
NNNCAGAAAGCAGGTGAACG |
NGG |
2 |
0.4333 |
Tier III |
| 8 |
NC_000022.11 |
22 |
34638285 |
+ |
LOC105373013 |
NNNCTCAAAGCAGGTGAATG |
NGG |
2 |
0.1385 |
Tier III |
| 9 |
NC_000004.12 |
4 |
28576425 |
+ |
LOC105374557 |
NNNCTCAAAGCAGGTGAAGG |
NGG |
2 |
0.0375 |
Tier III |
| 10 |
NC_000004.12 |
4 |
28576425 |
+ |
LOC105374558 |
NNNCTCAAAGCAGGTGAAGG |
NGG |
2 |
0.0375 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
43888982 |
+ |
Qrsl1 |
NNNCGCAAAGCAGGTGAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
108968038 |
+ |
Col7a1 |
NNNGGAAAAGCAGGTGAACG |
NGG |
2 |
0.4643 |
Tier I |
| 3 |
NC_000082.6 |
16 |
37086697 |
+ |
Polq |
NNNCGCAAAGCAGGTGAACC |
NAG |
2 |
0.1111 |
Tier I |
| 4 |
NC_000071.6 |
5 |
96740660 |
- |
Fras1 |
NNNGGCAAAGCACGTGAACG |
NGG |
2 |
0.2105 |
Tier II |
| 5 |
NC_000069.6 |
3 |
22076959 |
+ |
Tbl1xr1 |
NNNCGCCAAGCAGGTGAGCG |
NGG |
2 |
0.175 |
Tier II |
| 6 |
NC_000075.6 |
9 |
74849560 |
- |
Gm16551 |
NNNCACAAAGCAGGTGCACG |
NGG |
2 |
0.1529 |
Tier III |
| 7 |
NC_000075.6 |
9 |
74849560 |
- |
Gm33562 |
NNNCACAAAGCAGGTGCACG |
NGG |
2 |
0.1529 |
Tier III |
Other clones with same target sequence:
(none)
