Construct: sgRNA BRDN0001144734

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGATGCTGAGACATATACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK15 (65061)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76486

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 201823684 - CDK15 NNNATGCTGAGACATATACT NGG 0 1.0 Tier I
2 NC_000010.11 10 49843589 - PARG NNNATGCTGAGACATATCGT NGG 2 0.0238 Tier I
3 NC_000009.12 9 28771829 - LINGO2 NNNATACTGAGACATATTCT NGG 2 0.5 Tier II
4 NC_000005.10 5 129690985 - ADAMTS19 NNNATGCTGAAACATATTCT NGG 2 0.5 Tier II
5 NC_000008.11 8 49960651 + SNTG1 NNNATGCAGAGACATATAAT NGG 2 0.3429 Tier II
6 NC_000012.12 12 72266951 + TRHDE NNNATGCTTAGACAAATACT NGG 2 0.3088 Tier II
7 NC_000012.12 12 69852410 - MYRFL NNNATGCAGATACATATACT NGG 2 0.3077 Tier II
8 NC_000008.11 8 20271939 - LZTS1 NNNGTGCTGACACATATACT NGG 2 0.2679 Tier II
9 NC_000007.14 7 48520527 - ABCA13 NNNATGCTGAGACAAATAAT NGG 2 0.2481 Tier II
10 NC_000011.10 11 117693815 - DSCAML1 NNNAGGCTGAGAAATATACT NGG 2 0.2462 Tier II
11 NC_000003.12 3 119703872 + CFAP91 NNNGTGCTGATACATATACT NGG 2 0.2404 Tier II
12 NC_000019.10 19 54734024 - KIR3DL3 NNNCTGCTGAGACATATATT NGG 2 0.1629 Tier II
13 NC_000009.12 9 27371183 + MOB3B NNNATGCTGAGACACATAAT NGG 2 0.1169 Tier II
14 NC_000001.11 1 197181297 - ZBTB41 NNNATGCTGAGCCCTATACT NGG 2 0.0564 Tier II
15 NC_000007.14 7 73612858 + MLXIPL NNNATGCTGAGACAGATACT NGG 1 0.05 Tier II
16 NC_000007.14 7 89120913 + ZNF804B NNNATGCTGAGACAGATACT NGA 2 0.0035 Tier II
17 NC_000010.11 10 31891289 - ARHGAP12 NNNATGCTGAGAGATATACT NGC 2 0.003 Tier II
18 NC_000023.11 X 149547237 - EOLA1 NNNATGCTGAGACATTTACT NGC 2 0.0 Tier II
19 NC_000023.11 X 149932270 + EOLA2 NNNATGCTGAGACATTTACT NGC 2 0.0 Tier II
20 NC_000004.12 4 18226747 + LOC107986262 NNNATGCTGAAATATATACT NGG 2 0.7 Tier III
21 NC_000008.11 8 60069900 - LOC105375861 NNNATTCTTAGACATATACT NGG 2 0.3556 Tier III
22 NC_000008.11 8 54429683 - LOC105375841 NNNATTCTGAGACATATTCT NGG 2 0.3333 Tier III
23 NC_000012.12 12 72266951 + TRHDE-AS1 NNNATGCTTAGACAAATACT NGG 2 0.3088 Tier III
24 NC_000003.12 3 153652506 + LINC02006 NNNATGCTGAGACATACACT NAG 2 0.183 Tier III
25 NC_000001.11 1 156427480 - MIR9-1HG NNNATCCTGAGAGATATACT NGG 2 0.093 Tier III
26 NC_000002.12 2 34011412 + LINC01317 NNNATCCTGAGAGATATACT NGG 2 0.093 Tier III
27 NC_000010.11 10 52963686 + LOC105378308 NNNATGCTGAGACATATACT NTG 1 0.039 Tier III
28 NC_000010.11 10 57925418 + LOC124902547 NNNATGCTGAGACATTGACT NGG 2 0.0 Tier III
29 NC_000021.9 21 15417119 + LOC101927745 NNNATGCTGAGACATTTGCT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 59281428 - Cdk15 NNNATGCTGAGACATGTACT NGG 1 0.1923 Tier I
2 NC_000074.6 8 81919135 - Inpp4b NNNATGCAGGGACATATACT NGG 2 0.4444 Tier II
3 NC_000071.6 5 128073915 - Tmem132d NNNATGCAGAGACATATAAT NGG 2 0.3429 Tier II
4 NC_000080.6 14 57387907 + Cryl1 NNNGTGCTGAGACATAAACT NGG 2 0.3333 Tier II
5 NC_000067.6 1 6494388 + St18 NNNATGTTGAGACATATGCT NGG 2 0.325 Tier II
6 NC_000068.7 2 103727594 + Nat10 NNNAGGCTGAGACATATTCT NGG 2 0.32 Tier II
7 NC_000075.6 9 88354095 - Nt5e NNNATGCTGAGACATAAACA NGG 2 0.3 Tier II
8 NC_000068.7 2 15909648 - Malrd1 NNNATGCTGAGACTTATATT NGG 2 0.2462 Tier II
9 NC_000080.6 14 103681759 + Slain1 NNNATGCAGAGACATATACT NAG 2 0.2074 Tier II
10 NC_000070.6 4 123910678 + Mycbp NNNATGCTGAGATACATACT NGG 2 0.1909 Tier II
11 NC_000079.6 13 45804716 + Atxn1 NNNATGCTGACACATATGCT NGG 2 0.1714 Tier II
12 NC_000078.6 12 91410305 - Tshr NNNATACTGAGACATAGACT NGG 2 0.1176 Tier II
13 NC_000077.6 11 61036661 + Kcnj12 NNNATGCTGAGACATATAAT NAG 2 0.1111 Tier II
14 NC_000079.6 13 49510622 + Cenpp NNNATGCTGTGACATAGACT NGG 2 0.1038 Tier II
15 NC_000079.6 13 49510622 + Ecm2 NNNATGCTGTGACATAGACT NGG 2 0.1038 Tier II
16 NC_000068.7 2 120415125 - Ganc NNNATGCTTAGAGATATACT NGG 2 0.0727 Tier II
17 NC_000073.6 7 103920535 - Olfr631 NNNATGCAGAGACATATACT NTG 2 0.0312 Tier II
18 NC_000071.6 5 125558012 + Tmem132b NNNATGCTGAGACATAGACT NAG 2 0.0305 Tier II
19 NC_000071.6 5 48972269 + Kcnip4 NNNATGGTGAGACATATACT NTG 2 0.0183 Tier II
20 NC_000077.6 11 54736621 + Cdc42se2 NNNATGCTGAGACGTATACT NGT 2 0.0075 Tier II
21 NC_000073.6 7 89258754 - Tmem135 NNNATGCTCAGACATCTACT NGG 2 0.0 Tier II
22 NC_000076.6 10 63478545 + Ctnna3 NNNATGCTCAGACATTTACT NGG 2 0.0 Tier II
23 NC_000077.6 11 98251741 - Cdk12 NNNATGCTGAGACATATACT NAT 2 0.0 Tier II
24 NC_000070.6 4 123910678 + D130007C19Rik NNNATGCTGAGATACATACT NGG 2 0.1909 Tier III
25 NC_000078.6 12 7614449 + Gm32828 NNNATGCTGAGACCTATTCT NGG 2 0.1071 Tier III
26 NC_000070.6 4 23832433 - Gm31145 NNNAAGCTGAGACATGTACT NGG 2 0.0962 Tier III
27 NC_000069.6 3 62611972 + Gm34048 NNNATGCTGACACATATACT NTG 2 0.0167 Tier III
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Other clones with same target sequence:

(none)