Construct: sgRNA BRDN0001144740
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGAGCATGCACAACGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK18 (5129)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77880
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
205526833 |
+ |
CDK18 |
NNNGAGCATGCACAACGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
96297275 |
- |
CDK17 |
NNNGAGTATGCACAACGTAA |
NGG |
2 |
0.3482 |
Tier I |
| 3 |
NC_000010.11 |
10 |
96657512 |
+ |
PIK3AP1 |
NNNGAGCATGCACAGAGTCA |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000005.10 |
5 |
154333010 |
- |
GALNT10 |
NNNGAGCAGGCACAAAGTCA |
NGG |
2 |
0.619 |
Tier II |
| 5 |
NC_000005.10 |
5 |
50804046 |
+ |
PARP8 |
NNNGAGCATGCACACAGTCA |
NGG |
2 |
0.2727 |
Tier II |
| 6 |
NC_000012.12 |
12 |
75186338 |
+ |
KCNC2 |
NNNGAGCATGCACAAACTCA |
NGG |
2 |
0.2353 |
Tier II |
| 7 |
NC_000022.11 |
22 |
49647113 |
+ |
MIR3667HG |
NNNGACCATGCACAACCTCA |
NGG |
2 |
0.1604 |
Tier III |
| 8 |
NC_000020.11 |
20 |
24316608 |
- |
LOC105372577 |
NNNCAGCATGCACAACCTCA |
NGG |
2 |
0.1246 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
132118526 |
- |
Cdk18 |
NNNGAACATGCACAATGTCA |
NGG |
2 |
0.3077 |
Tier I |
| 2 |
NC_000076.6 |
10 |
93226771 |
+ |
Cdk17 |
NNNGAGCATGCACAATGTCA |
NGG |
1 |
0.3077 |
Tier I |
| 3 |
NC_000083.6 |
17 |
13855615 |
- |
Afdn |
NNNGAGCAGGCACAAAGTCA |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000073.6 |
7 |
29044027 |
+ |
Ryr1 |
NNNAAGCATGCACAACGTAA |
NGG |
2 |
0.3857 |
Tier II |
| 5 |
NC_000082.6 |
16 |
4169994 |
+ |
Crebbp |
NNNGAGCATGCACAAAGGCA |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000074.6 |
8 |
124712950 |
- |
Ttc13 |
NNNGAGCATGCACAAACTCA |
NGG |
2 |
0.2353 |
Tier II |
| 7 |
NC_000077.6 |
11 |
3178993 |
- |
Sfi1 |
NNNTAGCTTGCACAACGTCA |
NGG |
2 |
0.1558 |
Tier II |
| 8 |
NC_000068.7 |
2 |
130324181 |
- |
Ebf4 |
NNNTAGCATGCACAACGTCC |
NGG |
2 |
0.0826 |
Tier II |
| 9 |
NC_000068.7 |
2 |
157810061 |
- |
Ctnnbl1 |
NNNGAGCATGCACAAGGCCA |
NGG |
2 |
0.0659 |
Tier II |
| 10 |
NC_000068.7 |
2 |
130342418 |
- |
Ebf4 |
NNNGAGCATGCACAACCTCA |
NAG |
2 |
0.061 |
Tier II |
| 11 |
NC_000073.6 |
7 |
16795035 |
- |
Slc1a5 |
NNNTAGCATGCACAAGGTCA |
NGG |
2 |
0.0559 |
Tier II |
| 12 |
NC_000083.6 |
17 |
23795522 |
- |
Srrm2 |
NNNTAGCATGCACAAGGTCA |
NGG |
2 |
0.0559 |
Tier II |
| 13 |
NC_000083.6 |
17 |
71705355 |
- |
Togaram2 |
NNNTAGCATGCACAAGGTCA |
NGG |
2 |
0.0559 |
Tier II |
| 14 |
NC_000081.6 |
15 |
84962413 |
+ |
5031439G07Rik |
NNNGAGCATGGACAAGGTCA |
NGG |
2 |
0.0385 |
Tier II |
| 15 |
NC_000085.6 |
19 |
10727627 |
+ |
Cd5 |
NNNGAGCATGCACAAGCTCA |
NGG |
2 |
0.0362 |
Tier II |
| 16 |
NC_000079.6 |
13 |
24503066 |
- |
Ripor2 |
NNNGAGCATGCACAAGGTCC |
NGG |
2 |
0.035 |
Tier II |
| 17 |
NC_000081.6 |
15 |
59258048 |
- |
Gm36617 |
NNNCAGCATGCACAAAGTCA |
NGG |
2 |
0.5294 |
Tier III |
| 18 |
NC_000085.6 |
19 |
59511501 |
- |
2700089I24Rik |
NNNGAGCATGCCCAACGTCT |
NGG |
2 |
0.1579 |
Tier III |
| 19 |
NT_187064.1 |
|
5493 |
+ |
LOC100861749 |
NNNTAGCTTGCACAACGTCA |
NGG |
2 |
0.1558 |
Tier III |
| 20 |
NC_000083.6 |
17 |
23795522 |
- |
Gm52285 |
NNNTAGCATGCACAAGGTCA |
NGG |
2 |
0.0559 |
Tier III |
| 21 |
NC_000073.6 |
7 |
35834310 |
- |
E130304I02Rik |
NNNGAGCATGCACAAGGTCC |
NGG |
2 |
0.035 |
Tier III |
Other clones with same target sequence:
(none)
