Construct: sgRNA BRDN0001144744

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCACTGCAGCCCTTACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PINK1 (65018)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78038

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 20637942 - PINK1 NNNACTGCAGCCCTTACCAA NGG 0 1.0 Tier I
2 NC_000002.12 2 27461291 - IFT172 NNNACTGCAGCCCTTATCAA NGG 1 0.4667 Tier I
3 NC_000023.11 X 48818110 - HDAC6 NNNACTGCAGCCCTTACCTG NGG 2 0.4118 Tier I
4 NC_000004.12 4 71463576 + SLC4A4 NNNACTGTAGCACTTACCAA NGG 2 0.625 Tier II
5 NC_000001.11 1 53619436 + GLIS1 NNNACTACAGCCCTTACCAT NGG 2 0.6 Tier II
6 NC_000001.11 1 54269031 + SSBP3 NNNAGTTCAGCCCTTACCAA NGG 2 0.3429 Tier II
7 NC_000012.12 12 70540649 - PTPRB NNNAATTCAGCCCTTACCAA NGG 2 0.3265 Tier II
8 NC_000014.9 14 51544342 - FRMD6 NNNCCTGCATCCCTTACCAA NGG 2 0.2868 Tier II
9 NC_000010.11 10 16989953 + CUBN NNNAGTGCAGTCCTTACCAA NGG 2 0.1846 Tier II
10 NC_000017.11 17 33625448 + ASIC2 NNNACTGCAGCCCTAACCAA NAG 2 0.1501 Tier II
11 NC_000019.10 19 48361146 - TMEM143 NNNACTGCAGCCCTTACCAA NTG 1 0.039 Tier II
12 NC_000002.12 2 240001 - SH3YL1 NNNACTGCAGCCCTGACCAG NGG 2 0.0382 Tier II
13 NC_000009.12 9 137568860 - DPH7 NNNACTGGAGCCCTTAGCAA NGG 2 0.0378 Tier II
14 NC_000015.10 15 77495911 + HMG20A NNNACTGCAGCCCTCACGAA NGG 2 0.0364 Tier II
15 NC_000001.11 1 6031819 - KCNAB2 NNNACTGCAGCCCTGACCTA NGG 2 0.0269 Tier II
16 NC_000009.12 9 6471641 + UHRF2 NNNACTGCACCCCTGACCAA NGG 2 0.02 Tier II
17 NC_000009.12 9 93669609 - PHF2 NNNACTGCAGCCCTCACCAA NGC 2 0.0061 Tier II
18 NC_000009.12 9 93669609 - LOC124902215 NNNACTGCAGCCCTCACCAA NGC 2 0.0061 Tier II
19 NC_000012.12 12 14917793 + ERP27 NNNACTGCAGCCCTGACCAA NGA 2 0.0035 Tier II
20 NC_000001.11 1 11291707 - UBIAD1 NNNACTGCAGCCGTTACCAA NGC 2 0.003 Tier II
21 NC_000002.12 2 168170543 - STK39 NNNACTGCAGCCCTTCCGAA NGG 2 0.0 Tier II
22 NC_000012.12 12 70540649 - PTPRB-AS1 NNNAATTCAGCCCTTACCAA NGG 2 0.3265 Tier III
23 NC_000014.9 14 51544342 - FRMD6-AS2 NNNCCTGCATCCCTTACCAA NGG 2 0.2868 Tier III
24 NC_000009.12 9 115226520 + DELEC1 NNNACTGCAGCCCTCACAAA NGG 2 0.1469 Tier III
25 NC_000005.10 5 33427696 - TARS1-DT NNNACTGCAGCCCCTACCGA NGG 2 0.1071 Tier III
26 NC_000004.12 4 169308320 + LOC105377529 NNNACTGCAGCCCTTACCAG NGA 2 0.0531 Tier III
27 NC_000016.10 16 48737167 - LOC105371240 NNNACTGCAGCCCTTGCCAA NAG 2 0.0499 Tier III
28 NC_000004.12 4 159655560 - LOC107986324 NNNACTGCAGCCCTGACCAA NAG 2 0.013 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 75520935 - Scarf1 NNNGCTGCAGCCCTTCCCAA NGG 2 0.0 Tier I
2 NC_000074.6 8 107460972 - Wwp2 NNNACAGCATCCCTTACCAA NGG 2 0.7042 Tier II
3 NC_000073.6 7 99022858 + Uvrag NNNACTGCAGCCCTTACTAA NGG 1 0.6429 Tier II
4 NC_000081.6 15 103511329 - Pde1b NNNACTCCAGCCCTTAACAA NGG 2 0.3208 Tier II
5 NC_000068.7 2 143581201 - Pcsk2 NNNACTGCAGTACTTACCAA NGG 2 0.2198 Tier II
6 NC_000068.7 2 26280097 + Ccdc187 NNNACTCCAGCCCCTACCAA NGG 2 0.1964 Tier II
7 NC_000080.6 14 93466557 - Pcdh9 NNNACTGCAGCCCACACCAA NGG 2 0.1688 Tier II
8 NC_000079.6 13 13468107 + Nid1 NNNACAGCAGCCCTTGCCAA NGG 2 0.1667 Tier II
9 NC_000068.7 2 7242175 - Celf2 NNNTCTGCAGCCCTTACCAA NAG 2 0.165 Tier II
10 NC_000076.6 10 86806697 + Nt5dc3 NNNACTGCAGCCTTTACCCA NGG 2 0.1448 Tier II
11 NC_000072.6 6 58901966 - Herc3 NNNACTGCAGCCCTCACCGA NGG 2 0.1023 Tier II
12 NC_000070.6 4 87054586 + Slc24a2 NNNACTGCAGCCATTACCAC NGG 2 0.0874 Tier II
13 NC_000083.6 17 73345060 - Capn13 NNNACTGCAGCCCTCACCAC NGG 2 0.062 Tier II
14 NC_000081.6 15 71542485 - Fam135b NNNACTGCAGCACTTACCAA NGT 2 0.0115 Tier II
15 NC_000073.6 7 46948291 + Uevld NNNACTGCAGCCCTTACCAA NAA 2 0.0 Tier II
16 NC_000081.6 15 28188819 - Dnah5 NNNACTGCAGCCCTTACCAA NCA 2 0.0 Tier II
17 NC_000069.6 3 139227352 + Stpg2 NNNACTGCAGCCCTTCCTAA NGG 2 0.0 Tier II
18 NC_000084.6 18 80596734 + Gm41790 NNNACTGCAGCCCTAACAAA NGG 2 0.3117 Tier III
19 NC_000078.6 12 107727293 + 4930465M20Rik NNNACTGCTGCCCTTAACAA NGG 2 0.28 Tier III
20 NC_000077.6 11 89375120 - Gm42088 NNNACTGCAGCCCTTACCAA NAA 2 0.0 Tier III
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Other clones with same target sequence:

(none)