Construct: sgRNA BRDN0001144748
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTATTCCATATAGTGTCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NMRK1 (54981)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75829
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
75069807 |
+ |
NMRK1 |
NNNTTCCATATAGTGTCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
189219605 |
- |
TPRG1 |
NNNTTCCATAAAGTGTCAAG |
NTG |
2 |
0.0292 |
Tier I |
| 3 |
NC_000004.12 |
4 |
82673143 |
- |
SCD5 |
NNNTTCCATATAATGTCAAA |
NGG |
2 |
0.8654 |
Tier II |
| 4 |
NC_000005.10 |
5 |
41976964 |
- |
FBXO4 |
NNNTTCCATATAGTGTTAAG |
NGG |
1 |
0.4667 |
Tier II |
| 5 |
NC_000001.11 |
1 |
242440886 |
- |
PLD5 |
NNNATCCATACAGTGTCAAG |
NGG |
2 |
0.3175 |
Tier II |
| 6 |
NC_000005.10 |
5 |
94827065 |
+ |
MCTP1 |
NNNTTCCTTATAGTGTCAAT |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000012.12 |
12 |
122082784 |
- |
MLXIP |
NNNTTCCTTACAGTGTCAAG |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000002.12 |
2 |
214356374 |
- |
SPAG16 |
NNNTTCCATATTGTGTCAAT |
NGG |
2 |
0.2333 |
Tier II |
| 9 |
NC_000012.12 |
12 |
2817555 |
- |
ITFG2 |
NNNTTCCATAAAGTGTCAAG |
NAG |
2 |
0.1944 |
Tier II |
| 10 |
NC_000002.12 |
2 |
77293017 |
+ |
LRRTM4 |
NNNTACCATATTGTGTCAAG |
NGG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000001.11 |
1 |
212036851 |
- |
DTL |
NNNTTCCACATAGTTTCAAG |
NGG |
2 |
0.1319 |
Tier II |
| 12 |
NC_000011.10 |
11 |
126963564 |
- |
KIRREL3 |
NNNTTCCATATAGATTCAAG |
NGG |
2 |
0.0884 |
Tier II |
| 13 |
NC_000003.12 |
3 |
159784405 |
- |
SCHIP1 |
NNNTTCCTTATAGTGTCAAG |
NTG |
2 |
0.0167 |
Tier II |
| 14 |
NC_000003.12 |
3 |
159784405 |
- |
IQCJ-SCHIP1 |
NNNTTCCTTATAGTGTCAAG |
NTG |
2 |
0.0167 |
Tier II |
| 15 |
NC_000003.12 |
3 |
155943842 |
+ |
GMPS |
NNNTTGCATATAGTGTCAAG |
NGT |
2 |
0.0081 |
Tier II |
| 16 |
NC_000014.9 |
14 |
74528281 |
- |
LTBP2 |
NNNTTCCATATGGGGTCAAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000007.14 |
7 |
137292853 |
+ |
PTN |
NNNTTCCATTTAGGGTCAAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000003.12 |
3 |
171955949 |
- |
LOC105374217 |
NNNTTCCATATAGAGTCAAG |
NAG |
2 |
0.1605 |
Tier III |
| 19 |
NC_000011.10 |
11 |
34838914 |
- |
LOC102723568 |
NNNTTCCATATAGTGGCAAG |
NTG |
2 |
0.0135 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
32841136 |
- |
Nampt |
NNNTTCAATACAGTGTCAAG |
NGG |
2 |
0.5 |
Tier I |
| 2 |
NC_000085.6 |
19 |
18641410 |
- |
Nmrk1 |
NNNTTCCATATGGTGTCCAG |
NGG |
2 |
0.1376 |
Tier I |
| 3 |
NC_000076.6 |
10 |
33579600 |
- |
Clvs2 |
NNNTTACATATAGTGTTAAG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000078.6 |
12 |
76214755 |
- |
Tex21 |
NNNTTCCATATTGTTTCAAG |
NGG |
2 |
0.0476 |
Tier II |
| 5 |
NC_000078.6 |
12 |
41188431 |
+ |
Immp2l |
NNNTTCCATATAGAGTCAAG |
NGA |
2 |
0.043 |
Tier II |
| 6 |
NC_000085.6 |
19 |
38890075 |
+ |
Tbc1d12 |
NNNTTCCAGATAGTGTCAAG |
NTG |
2 |
0.0241 |
Tier II |
| 7 |
NC_000075.6 |
9 |
116664874 |
+ |
Rbms3 |
NNNTTCCATATTGTGTCAAG |
NTG |
2 |
0.013 |
Tier II |
| 8 |
NC_000068.7 |
2 |
145878125 |
+ |
Rin2 |
NNNTTCCAGATAGTGTCAAG |
NGT |
2 |
0.01 |
Tier II |
Other clones with same target sequence:
(none)
