Construct: sgRNA BRDN0001144749
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTATATTCCTTCTAACTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FRK (2444)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76240
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
116060002 |
- |
FRK |
NNNTATTCCTTCTAACTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
121593466 |
+ |
SORL1 |
NNNTACTCCTTCTAACAACG |
NGG |
2 |
0.4848 |
Tier II |
| 3 |
NC_000011.10 |
11 |
115238198 |
- |
CADM1 |
NNNTATTACTTCTAACTACC |
NGG |
2 |
0.2786 |
Tier II |
| 4 |
NC_000002.12 |
2 |
79242466 |
+ |
CTNNA2 |
NNNTATTCCTTCTAACTTTG |
NGG |
2 |
0.2308 |
Tier II |
| 5 |
NC_000009.12 |
9 |
125632444 |
+ |
MAPKAP1 |
NNNTTTTCCTTCTAACTACC |
NGG |
2 |
0.1558 |
Tier II |
| 6 |
NC_000018.10 |
18 |
76919518 |
+ |
ZNF236 |
NNNTATTCCTTCTATCTAAG |
NGG |
2 |
0.0857 |
Tier II |
| 7 |
NC_000014.9 |
14 |
37602015 |
- |
TTC6 |
NNNTTTTCCTTCTCACTACG |
NGG |
2 |
0.0779 |
Tier II |
| 8 |
NC_000010.11 |
10 |
126218319 |
+ |
ADAM12 |
NNNTATTCCTTCTAACTATG |
NTG |
2 |
0.018 |
Tier II |
| 9 |
NC_000013.11 |
13 |
72930477 |
- |
PIBF1 |
NNNTATTCCTTCTAACTATG |
NTG |
2 |
0.018 |
Tier II |
| 10 |
NC_000004.12 |
4 |
36288994 |
+ |
DTHD1 |
NNNTATTCCTTCTAACTATG |
NGT |
2 |
0.0074 |
Tier II |
| 11 |
NC_000015.10 |
15 |
37944385 |
+ |
TMCO5A |
NNNTATTCCTTCTAACTATG |
NGT |
2 |
0.0074 |
Tier II |
| 12 |
NC_000005.10 |
5 |
34010801 |
- |
C1QTNF3-AMACR |
NNNTATTCCTTCTAACTACA |
NGT |
2 |
0.0151 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
122765941 |
+ |
Pcca |
NNNTATTCCTTCTTACCACG |
NGG |
2 |
0.3765 |
Tier II |
| 2 |
NC_000075.6 |
9 |
104107319 |
- |
Acad11 |
NNNCATTCCTTCTAACAACG |
NGG |
2 |
0.3451 |
Tier II |
| 3 |
NC_000075.6 |
9 |
104107319 |
- |
Ackr4 |
NNNCATTCCTTCTAACAACG |
NGG |
2 |
0.3451 |
Tier II |
| 4 |
NC_000074.6 |
8 |
119012706 |
- |
Cdh13 |
NNNTATTCCTTCTAACTCCA |
NGG |
2 |
0.1786 |
Tier II |
| 5 |
NC_000072.6 |
6 |
113463660 |
+ |
Il17re |
NNNTATACCTTCTAACTCCG |
NGG |
2 |
0.1667 |
Tier II |
| 6 |
NT_165789.2 |
X |
327829 |
- |
Tmlhe |
NNNAATTCCTTCTACCTACG |
NGG |
2 |
0.1299 |
Tier II |
| 7 |
NC_000071.6 |
5 |
111162256 |
+ |
Ttc28 |
NNNAATTCCTTCTCACTACG |
NGG |
2 |
0.102 |
Tier II |
| 8 |
NC_000074.6 |
8 |
27453090 |
- |
Poteg |
NNNTATTCCTTCTAACCACG |
NTG |
2 |
0.0275 |
Tier II |
| 9 |
NC_000069.6 |
3 |
126655327 |
- |
Camk2d |
NNNTCTTCCTTCTAACTACG |
NTG |
2 |
0.0195 |
Tier II |
| 10 |
NC_000067.6 |
1 |
163576126 |
- |
Gm32333 |
NNNTATTCCTACTAACTATG |
NGG |
2 |
0.3462 |
Tier III |
Other clones with same target sequence:
(none)
