Construct: sgRNA BRDN0001144753
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACATCATCACTTTCAAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K12 (7786)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76760
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000012.12 |
12 |
53486439 |
- |
MAP3K12 |
NNNATCATCACTTTCAAGTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000007.14 |
7 |
94664600 |
- |
PEG10 |
NNNAGCATCACTTGCAAGTG |
NGG |
2 |
0.0 |
Tier I |
3 |
NC_000020.11 |
20 |
56482143 |
- |
RTF2 |
NNNATCATCAATTTCAAGTG |
NGG |
1 |
0.75 |
Tier II |
4 |
NC_000021.9 |
21 |
37148993 |
- |
TTC3 |
NNNATCAAAACTTTCAAGTG |
NGG |
2 |
0.6857 |
Tier II |
5 |
NC_000016.10 |
16 |
10571457 |
+ |
EMP2 |
NNNATCATTACTTTCAGGTG |
NGG |
2 |
0.1544 |
Tier II |
6 |
NC_000007.14 |
7 |
95491825 |
- |
ASB4 |
NNNATCATCACTTTAAAATG |
NGG |
2 |
0.1538 |
Tier II |
7 |
NC_000002.12 |
2 |
153082386 |
- |
GALNT13 |
NNNATCCTCCCTTTCAAGTG |
NGG |
2 |
0.1458 |
Tier II |
8 |
NC_000023.11 |
X |
29951346 |
+ |
IL1RAPL1 |
NNNATCATCACTTCCAACTG |
NGG |
2 |
0.1361 |
Tier II |
9 |
NC_000001.11 |
1 |
22658379 |
+ |
C1QB |
NNNATCATCACTTCCAAGAG |
NGG |
2 |
0.0816 |
Tier II |
10 |
NC_000003.12 |
3 |
124677260 |
- |
KALRN |
NNNATCATCAGTTTCAGGTG |
NGG |
2 |
0.0441 |
Tier II |
11 |
NC_000006.12 |
6 |
114329200 |
+ |
HS3ST5 |
NNNAGCATCACTTTGAAGTG |
NGG |
2 |
0.032 |
Tier II |
12 |
NC_000011.10 |
11 |
99959231 |
+ |
CNTN5 |
NNNATCATCAATTTCAAGTG |
NGC |
2 |
0.0167 |
Tier II |
13 |
NC_000002.12 |
2 |
9952982 |
- |
GRHL1 |
NNNATCATCACTTTCAGGTG |
NGC |
2 |
0.0039 |
Tier II |
14 |
NC_000019.10 |
19 |
56394553 |
- |
ZNF582-DT |
NNNATCCTCACTTTCAAATG |
NGG |
2 |
0.3029 |
Tier III |
15 |
NC_000011.10 |
11 |
33903269 |
- |
LOC105376621 |
NNNTTCATCACTTTCAAGTC |
NGG |
2 |
0.2727 |
Tier III |
16 |
NC_000002.12 |
2 |
52268691 |
+ |
NRXN1-DT |
NNNATCATCAGTTTCAAGTA |
NGG |
2 |
0.2344 |
Tier III |
17 |
NC_000001.11 |
1 |
187252980 |
- |
LINC01036 |
NNNATAATCACTTTCAAGTG |
NGA |
2 |
0.0645 |
Tier III |
18 |
NC_000008.11 |
8 |
133895351 |
+ |
LOC101927822 |
NNNAACATCACTTTCAAGTG |
NGA |
2 |
0.0347 |
Tier III |
19 |
NC_000006.12 |
6 |
114329200 |
+ |
HDAC2-AS2 |
NNNAGCATCACTTTGAAGTG |
NGG |
2 |
0.032 |
Tier III |
20 |
NC_000001.11 |
1 |
34631964 |
+ |
LOC105378641 |
NNNATCATCACTTTCATGTG |
NGT |
2 |
0.0022 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000081.6 |
15 |
102504470 |
- |
Map3k12 |
NNNATCATCACTTTCAAGTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000071.6 |
5 |
148524887 |
+ |
Ubl3 |
NNNATCATAACTCTCAAGTG |
NGG |
2 |
0.6767 |
Tier II |
3 |
NC_000068.7 |
2 |
73380179 |
- |
Gpr155 |
NNNATTATCACTTTCAAATG |
NGG |
2 |
0.6429 |
Tier II |
4 |
NC_000076.6 |
10 |
103200082 |
+ |
Lrriq1 |
NNNGTCATCACTTTCAAATG |
NGG |
2 |
0.4327 |
Tier II |
5 |
NC_000068.7 |
2 |
25911670 |
+ |
Kcnt1 |
NNNCTCATCACCTTCAAGTG |
NGG |
2 |
0.3344 |
Tier II |
6 |
NC_000077.6 |
11 |
55335536 |
- |
Fat2 |
NNNAGCCTCACTTTCAAGTG |
NGG |
2 |
0.28 |
Tier II |
7 |
NC_000067.6 |
1 |
153467666 |
- |
Dhx9 |
NNNTTCTTCACTTTCAAGTG |
NGG |
2 |
0.2784 |
Tier II |
8 |
NC_000072.6 |
6 |
12730231 |
- |
Thsd7a |
NNNATCATCACTTTCAAGCA |
NGG |
2 |
0.2586 |
Tier II |
9 |
NC_000076.6 |
10 |
112360090 |
+ |
Kcnc2 |
NNNATGATCACTTTCACGTG |
NGG |
2 |
0.0882 |
Tier II |
10 |
NC_000073.6 |
7 |
18718812 |
- |
Psg22 |
NNNATCATCACTTTCAAGTG |
NGA |
1 |
0.0694 |
Tier II |
11 |
NC_000073.6 |
7 |
18797761 |
+ |
Psg19 |
NNNATCATTACTTTCAAGTG |
NGA |
2 |
0.0608 |
Tier II |
12 |
NC_000080.6 |
14 |
34471433 |
+ |
Bmpr1a |
NNNTTCATCACTTTCAAGTG |
NTG |
2 |
0.0248 |
Tier II |
13 |
NC_000068.7 |
2 |
82220042 |
+ |
Zfp804a |
NNNATGATCACTTTCAAGTG |
NTG |
2 |
0.0195 |
Tier II |
14 |
NC_000083.6 |
17 |
12295465 |
+ |
Map3k4 |
NNNATTATCACTTTCAAGTG |
NGT |
2 |
0.015 |
Tier II |
15 |
NC_000068.7 |
2 |
33794736 |
- |
Mvb12b |
NNNATCATCACTTACAAGTG |
NGC |
2 |
0.0138 |
Tier II |
16 |
NC_000075.6 |
9 |
104786724 |
- |
Cpne4 |
NNNAACATCACTTTCAAGTG |
NGT |
2 |
0.0081 |
Tier II |
17 |
NC_000079.6 |
13 |
14131558 |
+ |
Arid4b |
NNNATCATCACTGTCAAGTG |
NGT |
2 |
0.0042 |
Tier II |
18 |
NC_000078.6 |
12 |
40464951 |
+ |
Dock4 |
NNNATCATCACTTTCCAGTG |
NGT |
2 |
0.0 |
Tier II |
19 |
NC_000083.6 |
17 |
34951020 |
+ |
1110038B12Rik |
NNNATCATCACTCTCAATTG |
NGG |
2 |
0.5263 |
Tier III |
20 |
NC_000077.6 |
11 |
19066233 |
+ |
4933406G16Rik |
NNNATCATGACTTTCAAGTT |
NGG |
2 |
0.4333 |
Tier III |
21 |
NC_000079.6 |
13 |
97467782 |
+ |
Lncenc1 |
NNNGACATCACTTTCAAGTG |
NGG |
2 |
0.3125 |
Tier III |
22 |
NC_000072.6 |
6 |
54355089 |
+ |
9130019P16Rik |
NNNATCATCACTGCCAAGTG |
NGG |
2 |
0.0745 |
Tier III |
23 |
NC_000072.6 |
6 |
93025323 |
- |
9530026P05Rik |
NNNATCAGCACTTTCAAGTG |
NGA |
2 |
0.0509 |
Tier III |
24 |
NC_000068.7 |
2 |
33794736 |
- |
Gm33856 |
NNNATCATCACTTACAAGTG |
NGC |
2 |
0.0138 |
Tier III |
25 |
NC_000067.6 |
1 |
61342171 |
+ |
LOC105243939 |
NNNATCACCACTTTCCAGTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)