Construct: sgRNA BRDN0001144763
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACGTGCGAAAACAAGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATR (545)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77549
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
142550221 |
- |
ATR |
NNNCGTGCGAAAACAAGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
6035582 |
- |
ANKRD61 |
NNNCGTGCGCACACAAGATG |
NGG |
2 |
0.0877 |
Tier I |
| 3 |
NC_000012.12 |
12 |
55773574 |
+ |
SARNP |
NNNCGTGCCAAAACAAGATC |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000011.10 |
11 |
123634265 |
+ |
SCN3B |
NNNCGTGGGAACACAAGATG |
NGG |
2 |
0.1692 |
Tier II |
| 5 |
NC_000007.14 |
7 |
6035582 |
- |
EIF2AK1 |
NNNCGTGCGCACACAAGATG |
NGG |
2 |
0.0877 |
Tier II |
| 6 |
NC_000010.11 |
10 |
127358899 |
+ |
DOCK1 |
NNNCGTGAGAAAACAAGATG |
NTG |
2 |
0.0253 |
Tier II |
| 7 |
NC_000023.11 |
X |
131746450 |
- |
FIRRE |
NNNCGTGTGAAAACAAAATG |
NGG |
2 |
0.8167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
133681577 |
+ |
Pcdh19 |
NNNCATGCGAAAACAAGCTG |
NGG |
2 |
0.1651 |
Tier II |
Other clones with same target sequence:
(none)
