Construct: sgRNA BRDN0001144776
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGATTGATGAAAGAACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK3 (23387)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75754
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
116862279 |
- |
SIK3 |
NNNGATTGATGAAAGAACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
115266648 |
- |
CADM1 |
NNNGAATGATAAAAGAACCC |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000004.12 |
4 |
94819542 |
- |
BMPR1B |
NNNGGCTGATGAAAGAACCC |
NGG |
2 |
0.6545 |
Tier II |
| 4 |
NC_000020.11 |
20 |
38769071 |
- |
ACTR5 |
NNNGAATGATGAGAGAACCC |
NGG |
2 |
0.5652 |
Tier II |
| 5 |
NC_000015.10 |
15 |
91975866 |
+ |
SLCO3A1 |
NNNGAGTGAGGAAAGAACCC |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000007.14 |
7 |
36720038 |
- |
AOAH |
NNNGCTTGCTGAAAGAACCC |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000006.12 |
6 |
106079388 |
- |
PRDM1 |
NNNGATAGATGAAACAACCC |
NGG |
2 |
0.2386 |
Tier II |
| 8 |
NC_000008.11 |
8 |
40686927 |
- |
ZMAT4 |
NNNGATTGAGGAAAGAACAC |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000010.11 |
10 |
14328143 |
- |
FRMD4A |
NNNGTTTGATTAAAGAACCC |
NGG |
2 |
0.1399 |
Tier II |
| 10 |
NC_000014.9 |
14 |
50319457 |
+ |
DMAC2L |
NNNGATTGAGGAAAGGACCC |
NGG |
2 |
0.0962 |
Tier II |
| 11 |
NC_000014.9 |
14 |
54747450 |
- |
SAMD4A |
NNNGATTGATGAAAGAGGCC |
NGG |
2 |
0.0235 |
Tier II |
| 12 |
NC_000005.10 |
5 |
114283088 |
- |
KCNN2 |
NNNGATTGATGAAAGAAACC |
NGT |
2 |
0.0087 |
Tier II |
| 13 |
NC_000006.12 |
6 |
72856765 |
+ |
KCNQ5 |
NNNGATTGATGAAAGTGCCC |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000008.11 |
8 |
101388081 |
+ |
LINC02845 |
NNNGATATATGAAAGAACCC |
NGG |
2 |
0.5469 |
Tier III |
| 15 |
NC_000021.9 |
21 |
36096681 |
+ |
CBR1-AS1 |
NNNGATTGATGAAAGGAGCC |
NGG |
2 |
0.0256 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
132915604 |
- |
Lrrn2 |
NNNGATTGATGAAGGAACCA |
NGG |
2 |
0.2333 |
Tier II |
| 2 |
NC_000079.6 |
13 |
16858456 |
- |
Sugct |
NNNGATTAATGACAGAACCC |
NGG |
2 |
0.2105 |
Tier II |
| 3 |
NC_000071.6 |
5 |
135813633 |
- |
Srrm3 |
NNNCATTGATGATAGAACCC |
NGG |
2 |
0.1588 |
Tier II |
| 4 |
NC_000072.6 |
6 |
126142228 |
+ |
Ntf3 |
NNNGATTGATAAAAGATCCC |
NGG |
2 |
0.1333 |
Tier II |
| 5 |
NC_000077.6 |
11 |
108199854 |
- |
Prkca |
NNNGTTTGATGAAAGAACCC |
NAG |
2 |
0.0943 |
Tier II |
| 6 |
NC_000073.6 |
7 |
75579453 |
- |
Akap13 |
NNNTATTGATGAAAGAACCC |
NAG |
2 |
0.0943 |
Tier II |
| 7 |
NC_000075.6 |
9 |
28420428 |
+ |
Opcml |
NNNCATTGATGAAAGAACCC |
NGA |
2 |
0.0368 |
Tier II |
| 8 |
NC_000082.6 |
16 |
75115985 |
+ |
Robo2 |
NNNGTTTGATGAAAGAACCC |
NGA |
2 |
0.0253 |
Tier II |
| 9 |
NC_000067.6 |
1 |
24074620 |
+ |
Fam135a |
NNNGATTGATGAATGAACCC |
NGC |
2 |
0.0119 |
Tier II |
| 10 |
NC_000067.6 |
1 |
127842618 |
+ |
Map3k19 |
NNNGATTGATGAAAGCACCC |
NGG |
1 |
0.0 |
Tier II |
| 11 |
NC_000078.6 |
12 |
99926757 |
- |
Tdp1 |
NNNGATTGATGAAATCACCC |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000070.6 |
4 |
10406482 |
+ |
Gm46879 |
NNNGATTGCTCAAAGAACCC |
NGG |
2 |
0.2449 |
Tier III |
| 13 |
NC_000067.6 |
1 |
132915604 |
- |
Gm31159 |
NNNGATTGATGAAGGAACCA |
NGG |
2 |
0.2333 |
Tier III |
| 14 |
NC_000072.6 |
6 |
52161454 |
- |
Hotairm1 |
NNNGATTGATGGAAGAACCC |
NGT |
2 |
0.0116 |
Tier III |
Other clones with same target sequence:
(none)
