Construct: sgRNA BRDN0001144778
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGAAGTAGCCATCTACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- JAK1 (3716)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76393
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
64860214 |
+ |
JAK1 |
NNNGAAGTAGCCATCTACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
67689745 |
- |
GFOD2 |
NNNGAAGTAACCATCCACCA |
NGG |
2 |
0.6222 |
Tier II |
| 3 |
NC_000012.12 |
12 |
96690917 |
+ |
CFAP54 |
NNNGAAGTATCCAACTACCA |
NGG |
2 |
0.503 |
Tier II |
| 4 |
NC_000006.12 |
6 |
131148887 |
- |
AKAP7 |
NNNGAGGTTGCCATCTACCA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000020.11 |
20 |
23367181 |
+ |
GZF1 |
NNNGAAGTTGGCATCTACCA |
NGG |
2 |
0.15 |
Tier II |
| 6 |
NC_000012.12 |
12 |
79250784 |
+ |
SYT1 |
NNNGAAGTAGCCACCTACCC |
NGG |
2 |
0.0649 |
Tier II |
| 7 |
NC_000001.11 |
1 |
114111300 |
- |
SYT6 |
NNNGTAGTAGCCATCTCCCA |
NGG |
2 |
0.0642 |
Tier II |
| 8 |
NC_000022.11 |
22 |
38243815 |
+ |
TMEM184B |
NNNGAAGTAGGCATCTCCCA |
NGG |
2 |
0.0441 |
Tier II |
| 9 |
NC_000022.11 |
22 |
39928684 |
+ |
GRAP2 |
NNNGAAGTAGCCATTTACCA |
NGA |
2 |
0.0046 |
Tier II |
| 10 |
NC_000001.11 |
1 |
202291459 |
+ |
LGR6 |
NNNGAAGCAGCCAGCTACCA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000014.9 |
14 |
105675027 |
+ |
IGH |
NNNGAAGTTGCCATCCACCA |
NGG |
2 |
0.4 |
Tier III |
| 12 |
NC_000020.11 |
20 |
52077837 |
- |
LOC105372664 |
NNNGAAGTAGCAATCTACAA |
NGG |
2 |
0.3061 |
Tier III |
| 13 |
NC_000001.11 |
1 |
212233861 |
+ |
LINC02608 |
NNNGACTTAGCCATCTACCA |
NGG |
2 |
0.2597 |
Tier III |
| 14 |
NC_000004.12 |
4 |
27986158 |
- |
LOC105374552 |
NNNGAAGTTGCCATCTACAA |
NGG |
2 |
0.2571 |
Tier III |
| 15 |
NC_000003.12 |
3 |
80997191 |
- |
LINC02027 |
NNNTAATTAGCCATCTACCA |
NGG |
2 |
0.2078 |
Tier III |
| 16 |
NC_000013.11 |
13 |
50175372 |
- |
DLEU1 |
NNNGAAGTAGCCAGCTTCCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
101175183 |
+ |
Jak1 |
NNNGAAGTAGCCATCCACCA |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000072.6 |
6 |
32210990 |
+ |
Plxna4 |
NNNGATGTAGCCATCCACCA |
NGG |
2 |
0.4762 |
Tier I |
| 3 |
NC_000075.6 |
9 |
21120369 |
+ |
Tyk2 |
NNNGAAATAGCCATCGACCA |
NGG |
2 |
0.3462 |
Tier I |
| 4 |
NC_000085.6 |
19 |
56594831 |
- |
Nhlrc2 |
NNNGAAGGAGCCATCTACCA |
NAG |
2 |
0.1901 |
Tier I |
| 5 |
NC_000082.6 |
16 |
13628218 |
+ |
Parn |
NNNAAAGCAGCCATCTACCA |
NGG |
2 |
0.9 |
Tier II |
| 6 |
NC_000067.6 |
1 |
32200689 |
+ |
Khdrbs2 |
NNNGGTGTAGCCATCTACCA |
NGG |
2 |
0.5143 |
Tier II |
| 7 |
NC_000072.6 |
6 |
141461654 |
+ |
Pde3a |
NNNCAAGTAGCCCTCTACCA |
NGG |
2 |
0.1115 |
Tier II |
| 8 |
NC_000086.7 |
X |
140419793 |
- |
Frmpd3 |
NNNGAAGTAGCCATATACTA |
NGG |
2 |
0.1026 |
Tier II |
| 9 |
NC_000080.6 |
14 |
29556150 |
- |
Cacna2d3 |
NNNGAAGTAGACATGTACCA |
NGG |
2 |
0.0375 |
Tier II |
| 10 |
NC_000076.6 |
10 |
108345589 |
- |
Pawr |
NNNGAAGTAGCCATCTGGCA |
NGG |
2 |
0.0235 |
Tier II |
| 11 |
NC_000079.6 |
13 |
41326307 |
+ |
Nedd9 |
NNNGAAGTAGCCATCTACCA |
NTC |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
