Construct: sgRNA BRDN0001144779
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAACGCTTCAGCCCAATTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SMG1 (23049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75769
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
18882202 |
- |
SMG1 |
NNNCGCTTCAGCCCAATTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
132606018 |
- |
INPP5A |
NNNCGCTCCAGCCCAATTCA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000011.10 |
11 |
133217641 |
+ |
OPCML |
NNNCTCTTCAGCCCAATTCA |
NGG |
2 |
0.2813 |
Tier II |
| 4 |
NC_000016.10 |
16 |
29551225 |
- |
SMG1P2 |
NNNCGCTTCAGCCCAATTCG |
NGT |
1 |
0.0161 |
Tier III |
| 5 |
NC_000016.10 |
16 |
30291239 |
- |
SMG1P5 |
NNNCGCTTCAGCCCAATTCG |
NGT |
1 |
0.0161 |
Tier III |
| 6 |
NC_000016.10 |
16 |
70243663 |
- |
SMG1P7 |
NNNCGCTTCAGCCCAATTCG |
NGT |
1 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
118198228 |
- |
Smg1 |
NNNCGCTTTAGCCCAATTCG |
NGG |
1 |
0.875 |
Tier I |
| 2 |
NC_000068.7 |
2 |
130241568 |
- |
Tmc2 |
NNNCACTTCAGCCCAGTTCG |
NGG |
2 |
0.1667 |
Tier I |
Other clones with same target sequence:
(none)
