Construct: sgRNA BRDN0001144783
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAAGGTGGGCCTAACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIPK4 (54101)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75970
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
41746632 |
+ |
RIPK4 |
NNNAAGGTGGGCCTAACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
113941991 |
+ |
HDAC2 |
NNNAAGGTGGCCCTAACTCT |
NGG |
2 |
0.3 |
Tier II |
| 3 |
NC_000006.12 |
6 |
136663849 |
- |
MAP3K5 |
NNNAAGGTGGGCCTCACTTG |
NGG |
2 |
0.1259 |
Tier II |
| 4 |
NC_000021.9 |
21 |
36216857 |
+ |
DOP1B |
NNNAAGGTGGGCCTCACTTG |
NGG |
2 |
0.1259 |
Tier II |
| 5 |
NC_000007.14 |
7 |
67305172 |
+ |
STAG3L4 |
NNNAAGGTGGGCCTCACTTG |
NGG |
2 |
0.1259 |
Tier III |
| 6 |
NC_000007.14 |
7 |
68180049 |
- |
LOC105375341 |
NNNAAGGTGGGCCTCACTTG |
NGG |
2 |
0.1259 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
77212967 |
- |
Nr3c2 |
NNNGAGGTGGGCCTAACTAG |
NGG |
2 |
0.2679 |
Tier II |
| 2 |
NC_000075.6 |
9 |
24490960 |
- |
Dpy19l1 |
NNNAAGGTGGGTCTAACTAG |
NGG |
2 |
0.2308 |
Tier II |
| 3 |
NC_000072.6 |
6 |
88309072 |
- |
Eefsec |
NNNAAGGTGGGCCTAAGTCA |
NGG |
2 |
0.0551 |
Tier II |
| 4 |
NC_000086.7 |
X |
107849303 |
- |
Tent5d |
NNNAAGGTGGGCCTAACTCT |
NTG |
2 |
0.0273 |
Tier II |
| 5 |
NC_000075.6 |
9 |
24490960 |
- |
LOC115487153 |
NNNAAGGTGGGTCTAACTAG |
NGG |
2 |
0.2308 |
Tier III |
Other clones with same target sequence:
(none)
