Construct: sgRNA BRDN0001144785
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAATATTTGACTTCTAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK3 (79646)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76295
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
168568864 |
- |
PANK3 |
NNNATATTTGACTTCTAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
26332063 |
+ |
TAF4B |
NNNATATTAGACTTCTAACT |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000003.12 |
3 |
151426680 |
- |
MED12L |
NNNATATTTGATTTCTAACA |
NGG |
2 |
0.5048 |
Tier II |
| 4 |
NC_000001.11 |
1 |
14422919 |
+ |
KAZN |
NNNCTATTTGACTTCTAACA |
NGG |
2 |
0.3309 |
Tier II |
| 5 |
NC_000001.11 |
1 |
20757700 |
+ |
HP1BP3 |
NNNACATTTGACTTCTAACG |
NTG |
2 |
0.039 |
Tier II |
| 6 |
NC_000003.12 |
3 |
114136058 |
+ |
DRD3 |
NNNATATTTGACTTCTAACG |
NAT |
2 |
0.0 |
Tier II |
| 7 |
NC_000007.14 |
7 |
12372103 |
+ |
VWDE |
NNNCTATTTGACTGCTAACG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000013.11 |
13 |
38651696 |
- |
LINC00437 |
NNNATATTTAACTTCTAACA |
NGG |
2 |
0.875 |
Tier III |
| 9 |
NC_000016.10 |
16 |
86480497 |
- |
FENDRR |
NNNTTATTTGACTTCTAACA |
NGG |
2 |
0.5966 |
Tier III |
| 10 |
NC_000004.12 |
4 |
25194961 |
- |
SEPSECS-AS1 |
NNNATATTTGACTTCAGACG |
NGG |
2 |
0.1604 |
Tier III |
| 11 |
NC_000004.12 |
4 |
25194961 |
- |
LOC105374535 |
NNNATATTTGACTTCAGACG |
NGG |
2 |
0.1604 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
35915495 |
+ |
Wwc1 |
NNNATATTTTACCTCTAACG |
NGG |
2 |
0.6414 |
Tier II |
| 2 |
NC_000083.6 |
17 |
30371351 |
- |
Btbd9 |
NNNAAATTCGACTTCTAACG |
NGG |
2 |
0.4615 |
Tier II |
| 3 |
NC_000074.6 |
8 |
127607117 |
+ |
Pard3 |
NNNATATTTAACTTCTAATG |
NGG |
2 |
0.4308 |
Tier II |
| 4 |
NC_000071.6 |
5 |
81042700 |
+ |
Adgrl3 |
NNNATATCTGACTTCTAAAG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000069.6 |
3 |
130198823 |
+ |
Col25a1 |
NNNATATTTGACTTCTAAAG |
NCG |
2 |
0.0459 |
Tier II |
| 6 |
NC_000079.6 |
13 |
112782097 |
- |
Gm41067 |
NNNATATTTGACTCCTAAAG |
NGG |
2 |
0.1224 |
Tier III |
| 7 |
NC_000070.6 |
4 |
78284666 |
- |
Gm32545 |
NNNATATTTGACTTCTAAGG |
NTG |
2 |
0.0049 |
Tier III |
| 8 |
NC_000082.6 |
16 |
77443669 |
+ |
Mir99ahg |
NNNATATTTGAATGCTAACG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
