Construct: sgRNA BRDN0001144791
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAACACTACCAGCTACCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IKBKE (9641)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77772
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
206474379 |
+ |
IKBKE |
NNNACACTACCAGCTACCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
234231322 |
- |
SLC35F3 |
NNNTCACCACCAGCTACCTG |
NGG |
2 |
0.6364 |
Tier II |
| 3 |
NC_000016.10 |
16 |
85582019 |
+ |
GSE1 |
NNNATACTGCCAGCTACCTG |
NGG |
2 |
0.4242 |
Tier II |
| 4 |
NC_000017.11 |
17 |
50096312 |
+ |
PDK2 |
NNNCCACTACCAGCAACCTG |
NGG |
2 |
0.2043 |
Tier II |
| 5 |
NC_000007.14 |
7 |
70065974 |
- |
AUTS2 |
NNNACAATACCAGCTACCTG |
NGA |
2 |
0.0521 |
Tier II |
| 6 |
NC_000010.11 |
10 |
119927027 |
- |
SEC23IP |
NNNACACTCCCAGCTACCTG |
NGA |
2 |
0.0397 |
Tier II |
| 7 |
NC_000001.11 |
1 |
196361666 |
- |
KCNT2 |
NNNACACCACCAGCTACCTG |
NTG |
2 |
0.039 |
Tier II |
| 8 |
NC_000014.9 |
14 |
41835617 |
+ |
LRFN5 |
NNNACAGTACCAGCTACCTG |
NGA |
2 |
0.0327 |
Tier II |
| 9 |
NC_000008.11 |
8 |
80788367 |
+ |
ZNF704 |
NNNACACTACCAGCTACCTG |
NTC |
2 |
0.0 |
Tier II |
| 10 |
NC_000020.11 |
20 |
3236885 |
+ |
SLC4A11 |
NNNACACTACCAGGTTCCTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000014.9 |
14 |
73775601 |
+ |
MIDEAS |
NNNACACTCCCAGCTTCCTG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000016.10 |
16 |
6546718 |
+ |
RBFOX1 |
NNNACATTACCAGGTACCTG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000015.10 |
15 |
100344669 |
- |
SPATA41 |
NNNACTCTACCAGCTACCTG |
NGG |
1 |
0.7143 |
Tier III |
| 14 |
NC_000009.12 |
9 |
11446841 |
+ |
LOC105375974 |
NNNATACTACCAGCTACCTG |
NGG |
1 |
0.6364 |
Tier III |
| 15 |
NC_000014.9 |
14 |
105042766 |
+ |
LOC124903398 |
NNNAGACCACCAGCTACCTG |
NGG |
2 |
0.6 |
Tier III |
| 16 |
NC_000009.12 |
9 |
73269984 |
- |
LOC101927281 |
NNNACACTATCAGATACCTG |
NGG |
2 |
0.3294 |
Tier III |
| 17 |
NC_000020.11 |
20 |
7235908 |
- |
LINC01428 |
NNNACACTCCCATCTACCTG |
NGG |
2 |
0.1714 |
Tier III |
| 18 |
NC_000001.11 |
1 |
196361666 |
- |
LOC124904597 |
NNNACACCACCAGCTACCTG |
NTG |
2 |
0.039 |
Tier III |
| 19 |
NC_000016.10 |
16 |
50900717 |
+ |
LINC02128 |
NNNACACCACCAGCTACCTG |
NTG |
2 |
0.039 |
Tier III |
| 20 |
NC_000004.12 |
4 |
184835778 |
- |
LOC105377587 |
NNNATACTACCAGCTCCCTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
77797170 |
- |
Myo18a |
NNNACACTTTCAGCTACCTG |
NGG |
2 |
0.5647 |
Tier II |
| 2 |
NC_000076.6 |
10 |
77937022 |
+ |
Trpm2 |
NNNACACGACCAGCTACCTC |
NGG |
2 |
0.3143 |
Tier II |
| 3 |
NC_000072.6 |
6 |
128016025 |
+ |
Tspan9 |
NNNCCACTGCCAGCTACCTG |
NGG |
2 |
0.2353 |
Tier II |
| 4 |
NC_000074.6 |
8 |
69825646 |
+ |
Lpar2 |
NNNACACTACCATTTACCTG |
NGG |
2 |
0.22 |
Tier II |
| 5 |
NC_000072.6 |
6 |
122964461 |
+ |
Clec4a3 |
NNNTCACTACCAGCTGCCTG |
NGG |
2 |
0.1224 |
Tier II |
| 6 |
NC_000079.6 |
13 |
24138808 |
- |
Carmil1 |
NNNACACTACCAGCTAGGTG |
NGG |
2 |
0.0078 |
Tier II |
| 7 |
NC_000074.6 |
8 |
120158286 |
+ |
6430548M08Rik |
NNNACACTACCAGCTGCCTG |
NGC |
2 |
0.0043 |
Tier II |
| 8 |
NC_000071.6 |
5 |
36266745 |
+ |
Sorcs2 |
NNNACACTACCAGCTTCCTG |
NGC |
2 |
0.0 |
Tier II |
| 9 |
NC_000079.6 |
13 |
98996792 |
+ |
Gm35279 |
NNNACACTACCATCCACCTG |
NGG |
2 |
0.0818 |
Tier III |
| 10 |
NC_000080.6 |
14 |
72124062 |
+ |
Gm41204 |
NNNACACTACCAGCCTCCTG |
NGG |
2 |
0.0 |
Tier III |
| 11 |
NC_000073.6 |
7 |
67837022 |
- |
Gm39027 |
NNNTCACTACCAGCTTCCTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
