Construct: sgRNA BRDN0001144796
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGAATGATATTGTCCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL5 (6792)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77198
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
18579964 |
+ |
CDKL5 |
NNNAATGATATTGTCCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
112997655 |
+ |
MCF2L |
NNNAATGAAATTGTCCATCC |
NGG |
2 |
0.398 |
Tier II |
| 3 |
NC_000005.10 |
5 |
78834873 |
+ |
ARSB |
NNNAATGTTATTGTCCATAG |
NGG |
2 |
0.1837 |
Tier II |
| 4 |
NC_000011.10 |
11 |
104159911 |
+ |
PDGFD |
NNNAATGATATTATCCATGG |
NGG |
2 |
0.1154 |
Tier II |
| 5 |
NC_000004.12 |
4 |
174822309 |
- |
GLRA3 |
NNNAATGATAATGTCCATGG |
NGG |
2 |
0.0938 |
Tier II |
| 6 |
NC_000002.12 |
2 |
13163569 |
- |
LOC105373436 |
NNNAATGATAGTGTCCACCG |
NGG |
2 |
0.1714 |
Tier III |
| 7 |
NC_000002.12 |
2 |
13163569 |
- |
LOC105373484 |
NNNAATGATAGTGTCCACCG |
NGG |
2 |
0.1714 |
Tier III |
| 8 |
NC_000003.12 |
3 |
195975378 |
- |
SDHAP1 |
NNNAATGATCTTGTACATCG |
NGG |
2 |
0.0741 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
77847167 |
- |
Pbsn |
NNNAGTGATATTATCCATCG |
NGG |
2 |
0.6646 |
Tier II |
| 2 |
NC_000084.6 |
18 |
84365554 |
- |
Zfp407 |
NNNAATGATGTTATCCATCG |
NGG |
2 |
0.5128 |
Tier II |
| 3 |
NC_000067.6 |
1 |
132675182 |
- |
Nfasc |
NNNAGTGATACTGTCCATCG |
NGG |
2 |
0.48 |
Tier II |
| 4 |
NC_000067.6 |
1 |
152803759 |
- |
Ncf2 |
NNNAATGATACTGTCCAGCG |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000073.6 |
7 |
64291484 |
+ |
Mtmr10 |
NNNAATCATATTGTCTATCG |
NGG |
2 |
0.2115 |
Tier II |
| 6 |
NC_000070.6 |
4 |
149647619 |
- |
Clstn1 |
NNNAATGATTTTGTACATCG |
NGG |
2 |
0.1961 |
Tier II |
| 7 |
NC_000067.6 |
1 |
132675182 |
- |
Gm46157 |
NNNAGTGATACTGTCCATCG |
NGG |
2 |
0.48 |
Tier III |
| 8 |
NC_000070.6 |
4 |
22958210 |
- |
Gm30886 |
NNNAAAGATATTGTCCATGG |
NGG |
2 |
0.1083 |
Tier III |
Other clones with same target sequence:
(none)
