Construct: sgRNA BRDN0001144797
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAACCAACATTTCGAGAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKI (9162)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76613
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
137654757 |
- |
DGKI |
NNNCCAACATTTCGAGAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
87894702 |
- |
NTRK3 |
NNNCCAACAGTTGGAGAAGG |
NGG |
2 |
0.0682 |
Tier II |
| 3 |
NC_000011.10 |
11 |
76977521 |
- |
ACER3 |
NNNCCAACATTTAGAGAAGG |
NTG |
2 |
0.015 |
Tier II |
| 4 |
NC_000008.11 |
8 |
136648304 |
+ |
LINC02055 |
NNNTCAACATTTGGAGAAGG |
NGG |
2 |
0.1091 |
Tier III |
| 5 |
NC_000011.10 |
11 |
76977521 |
- |
ACER3-AS1 |
NNNCCAACATTTAGAGAAGG |
NTG |
2 |
0.015 |
Tier III |
| 6 |
NC_000019.10 |
19 |
29297226 |
- |
VSTM2B-DT |
NNNCCAACATTTAGAGAAGG |
NTG |
2 |
0.015 |
Tier III |
| 7 |
NC_000001.11 |
1 |
29620814 |
- |
LOC107984934 |
NNNCCAACATTTAGATAAGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
37096273 |
- |
Dgki |
NNNCCAACATTTCGGGAAGG |
NGG |
1 |
0.65 |
Tier I |
| 2 |
NC_000074.6 |
8 |
78306092 |
+ |
Ttc29 |
NNNCAGACATTTCGAGAAGG |
NGG |
2 |
0.4082 |
Tier II |
| 3 |
NC_000074.6 |
8 |
77162708 |
+ |
Nr3c2 |
NNNCCAACAGTTCCAGAAGG |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000077.6 |
11 |
83363534 |
- |
Ap2b1 |
NNNCCAACATTTCTAGAATG |
NGG |
2 |
0.1778 |
Tier II |
| 5 |
NC_000072.6 |
6 |
111498449 |
+ |
Grm7 |
NNNCCAACATTTAGAGAAGG |
NTG |
2 |
0.015 |
Tier II |
| 6 |
NC_000081.6 |
15 |
51615806 |
+ |
Gm34562 |
NNNACAACATTTCAAGAAGG |
NGG |
2 |
0.6316 |
Tier III |
| 7 |
NC_000075.6 |
9 |
115506145 |
- |
Gm39447 |
NNNCCAACATTCCCAGAAGG |
NGG |
2 |
0.406 |
Tier III |
| 8 |
NC_000074.6 |
8 |
116268338 |
- |
Gm31278 |
NNNCCAGCATTTCGAGAAGC |
NGG |
2 |
0.3025 |
Tier III |
Other clones with same target sequence:
(none)
