Construct: sgRNA BRDN0001144803
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCTAGTGAAAGCTCCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK3 (85443)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77311
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
36738418 |
+ |
DCLK3 |
NNNCTAGTGAAAGCTCCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
169416033 |
- |
CCDC181 |
NNNCTACTGAAAACTCCTCG |
NGG |
2 |
0.6346 |
Tier II |
| 3 |
NC_000003.12 |
3 |
98734034 |
+ |
ST3GAL6 |
NNNCTGCTGAAAGCTCCTCG |
NGG |
2 |
0.4911 |
Tier II |
| 4 |
NC_000019.10 |
19 |
641792 |
- |
FGF22 |
NNNAAAGTGAAAGCTCCTCG |
NGG |
2 |
0.4211 |
Tier II |
| 5 |
NC_000014.9 |
14 |
77916373 |
- |
ADCK1 |
NNNCTAGTGAAAGCTTCTCT |
NGG |
2 |
0.2154 |
Tier II |
| 6 |
NC_000014.9 |
14 |
100530241 |
+ |
WDR25 |
NNNCTAGTGAAAGCGCCTGG |
NGG |
2 |
0.0063 |
Tier II |
| 7 |
NC_000016.10 |
16 |
52007448 |
- |
LINC02911 |
NNNCTAGTGAAAGCACCTCC |
NGG |
2 |
0.2481 |
Tier III |
| 8 |
NC_000003.12 |
3 |
117788719 |
- |
LOC101926953 |
NNNCTAGTGCAAGCTCCCCG |
NGG |
2 |
0.1429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
11221563 |
+ |
Prkn |
NNNCTAGTGAAAACTCCTCA |
NGG |
2 |
0.8654 |
Tier II |
| 2 |
NC_000075.6 |
9 |
90257594 |
- |
Tbc1d2b |
NNNCCAGTGAAAGCACCTCG |
NGG |
2 |
0.5789 |
Tier II |
| 3 |
NC_000074.6 |
8 |
22043746 |
- |
Atp7b |
NNNCTACTGAAAGCTCCTCT |
NGG |
2 |
0.4812 |
Tier II |
| 4 |
NC_000071.6 |
5 |
114302684 |
+ |
Myo1h |
NNNCTAGCGACAGCTCCTCG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000084.6 |
18 |
36431550 |
- |
Pfdn1 |
NNNCTAGTGAAAGCTTCTCG |
NGG |
1 |
0.3077 |
Tier II |
Other clones with same target sequence:
(none)
