Construct: sgRNA BRDN0001144804
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGACGACCCTACAGACACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKA1 (5255)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77839
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
72636345 |
- |
PHKA1 |
NNNACGACCCTACAGACACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
89116251 |
- |
ABHD2 |
NNNACCACCCTACAGACGCA |
NGG |
2 |
0.2727 |
Tier II |
| 3 |
NC_000001.11 |
1 |
94243865 |
+ |
ARHGAP29 |
NNNCAGACCCTACAGACACA |
NGG |
2 |
0.2017 |
Tier II |
| 4 |
NC_000019.10 |
19 |
41312657 |
- |
CCDC97 |
NNNACGACCCTGCAGACACC |
NGG |
2 |
0.1641 |
Tier II |
| 5 |
NC_000022.11 |
22 |
23227834 |
- |
BCR |
NNNACGACCCAACAGGCACA |
NGG |
2 |
0.1442 |
Tier II |
| 6 |
NC_000002.12 |
2 |
143434835 |
- |
ARHGAP15 |
NNNACGACCCTACAGAAACA |
NAG |
2 |
0.121 |
Tier II |
| 7 |
NC_000003.12 |
3 |
76882065 |
- |
ROBO2 |
NNNACGACCCAACAGACACA |
NTG |
2 |
0.0292 |
Tier II |
| 8 |
NC_000010.11 |
10 |
303001 |
- |
DIP2C |
NNNACGAACCTACAGACACA |
NGC |
2 |
0.0144 |
Tier II |
| 9 |
NC_000011.10 |
11 |
1392835 |
- |
BRSK2 |
NNNACGACCCTACAGCCACA |
NTG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
102575033 |
- |
Phka1 |
NNNACGACCCTACAGGCTCA |
NGG |
2 |
0.0962 |
Tier I |
| 2 |
NC_000076.6 |
10 |
81561345 |
- |
Tle5 |
NNNACAACTCTACAGACACA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000069.6 |
3 |
121447404 |
- |
Cnn3 |
NNNACAACCCTGCAGACACA |
NGG |
2 |
0.7222 |
Tier II |
| 4 |
NC_000067.6 |
1 |
89840257 |
+ |
Agap1 |
NNNAGAACCCTACAGACACA |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000075.6 |
9 |
115961747 |
- |
Gadl1 |
NNNACGACCCCACAGACACC |
NGG |
2 |
0.1515 |
Tier II |
| 6 |
NC_000084.6 |
18 |
53514810 |
- |
Prdm6 |
NNNACGACCCTACAGAAACA |
NCG |
2 |
0.05 |
Tier II |
| 7 |
NC_000079.6 |
13 |
59166545 |
- |
Gm34354 |
NNNACGACCCTGAAGACACA |
NGG |
2 |
0.2778 |
Tier III |
| 8 |
NC_000075.6 |
9 |
115961747 |
- |
Gm31234 |
NNNACGACCCCACAGACACC |
NGG |
2 |
0.1515 |
Tier III |
| 9 |
NC_000081.6 |
15 |
93194741 |
- |
Gm38535 |
NNNACGACCCTACAGACAGC |
NGG |
2 |
0.0284 |
Tier III |
Other clones with same target sequence:
(none)
