Construct: sgRNA BRDN0001144808
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTCACGTATCCGGCTCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA8 (2046)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76526
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
22569349 |
+ |
EPHA8 |
NNNCACGTATCCGGCTCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
128368829 |
- |
EEFSEC |
NNNCACGTGTCAGGCTCATG |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000002.12 |
2 |
3259533 |
+ |
EIPR1 |
NNNCACGTATCCGGTTCATG |
NAG |
2 |
0.0173 |
Tier II |
| 4 |
NC_000013.11 |
13 |
76816752 |
- |
LOC107984587 |
NNNCACGTGGCCGGCTCATG |
NGG |
2 |
0.3333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
136951770 |
- |
Epha8 |
NNNCACGTATCCCGCTCATG |
NGG |
1 |
0.4211 |
Tier I |
| 2 |
NC_000078.6 |
12 |
53347682 |
+ |
Npas3 |
NNNCACGTATCAGGCTCATG |
NGA |
2 |
0.0496 |
Tier II |
Other clones with same target sequence:
(none)
