Construct: sgRNA BRDN0001144811

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGTTCGACTTATTGAAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKK (139189)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75842

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 50393170 - DGKK NNNTTCGACTTATTGAAGGG NGG 0 1.0 Tier I
2 NC_000002.12 2 79324995 - CTNNA2 NNNTGAGACTTATTGAAGGG NGG 2 0.5943 Tier II
3 NC_000003.12 3 132615916 + ACAD11 NNNTATGACTTATTGAAGGG NGG 2 0.4643 Tier II
4 NC_000009.12 9 5942715 + BRD10 NNNTTCTACTTTTTGAAGGG NGG 2 0.1905 Tier II
5 NC_000003.12 3 132615916 + NPHP3-ACAD11 NNNTATGACTTATTGAAGGG NGG 2 0.4643 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 118346784 + Cdh13 NNNTTCCACTCATTGAAGGG NGG 2 0.4583 Tier II
2 NC_000083.6 17 42607620 - Opn5 NNNTTCCACTTATTCAAGGG NGG 2 0.1875 Tier II
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Other clones with same target sequence:

(none)