Construct: sgRNA BRDN0001144817
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATCTGACGGAAGACAACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MELK (9833)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75667
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
36594698 |
- |
MELK |
NNNCTGACGGAAGACAACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
1436012 |
- |
CCDC154 |
NNNCTAAAGGAAGACAACCC |
NGG |
2 |
0.65 |
Tier I |
| 3 |
NC_000010.11 |
10 |
13647491 |
- |
PRPF18 |
NNNCTCACGGAGGACAACCC |
NGG |
2 |
0.4924 |
Tier II |
| 4 |
NC_000010.11 |
10 |
13647491 |
- |
FRMD4A |
NNNCTCACGGAGGACAACCC |
NGG |
2 |
0.4924 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
44308976 |
- |
Melk |
NNNCTGACGGAAGACGACCC |
NGG |
1 |
0.1923 |
Tier I |
| 2 |
NC_000083.6 |
17 |
71510317 |
+ |
Ndc80 |
NNNCTGACGTGAGACAACCC |
NGG |
2 |
0.5281 |
Tier II |
| 3 |
NC_000082.6 |
16 |
81510347 |
- |
Ncam2 |
NNNCTGACGGAGGACAAACC |
NGG |
2 |
0.3889 |
Tier II |
| 4 |
NC_000071.6 |
5 |
93129474 |
+ |
Septin11 |
NNNGTGACTGAAGACAACCC |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000067.6 |
1 |
124133127 |
+ |
Dpp10 |
NNNCTGAAGGAAGACAGCCC |
NGG |
2 |
0.1147 |
Tier II |
| 6 |
NC_000083.6 |
17 |
35920602 |
+ |
Ppp1r10 |
NNNCTGAGGGAAGACAGCCC |
NGG |
2 |
0.1134 |
Tier II |
| 7 |
NC_000079.6 |
13 |
91986191 |
+ |
Rasgrf2 |
NNNCTGACTGAAGAGAACCC |
NGG |
2 |
0.0267 |
Tier II |
| 8 |
NC_000067.6 |
1 |
12429420 |
- |
Gm39591 |
NNNCTGACGGAAGACAAGAC |
NGG |
2 |
0.0571 |
Tier III |
Other clones with same target sequence:
(none)
