Construct: sgRNA BRDN0001144827
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTACAAGGTGTCCCCGCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA10 (284656)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76097
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
37754339 |
- |
EPHA10 |
NNNACAAGGTGTCCCCGCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
48122328 |
+ |
PTPRJ |
NNNACAAGGTGACCCCGAGG |
NGG |
2 |
0.4308 |
Tier II |
| 3 |
NC_000022.11 |
22 |
37396473 |
- |
ELFN2 |
NNNACAAGGTGTGCCCGCTG |
NGG |
2 |
0.0909 |
Tier II |
| 4 |
NC_000010.11 |
10 |
3651492 |
- |
LOC105376360 |
NNNACAAGGTGTCCCCCAGG |
NGG |
2 |
0.1267 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
124894124 |
+ |
Epha10 |
NNNACAAGGTGTCCCCAAGG |
NGG |
2 |
0.5026 |
Tier I |
| 2 |
NC_000077.6 |
11 |
87443112 |
+ |
Tex14 |
NNNAGAAGGTGTCCCCGCGA |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000077.6 |
11 |
87448502 |
+ |
Tex14 |
NNNAGAAGGTGTCCCCGCGA |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000077.6 |
11 |
87462161 |
+ |
Tex14 |
NNNAGAAGGTGTCCCCGCGA |
NGG |
2 |
0.5625 |
Tier II |
| 5 |
NC_000077.6 |
11 |
87471243 |
+ |
Tex14 |
NNNAGAAGGTGTCCCCGCGA |
NGG |
2 |
0.5625 |
Tier II |
| 6 |
NC_000075.6 |
9 |
76237264 |
- |
Hcrtr2 |
NNNTCAAGCTGTCCCCGCGG |
NGG |
2 |
0.3427 |
Tier II |
| 7 |
NC_000079.6 |
13 |
51848142 |
- |
Gadd45g |
NNNACAAGGTGTCCCCGCTG |
NCG |
2 |
0.0714 |
Tier II |
| 8 |
NC_000077.6 |
11 |
87443112 |
+ |
LOC115487798 |
NNNAGAAGGTGTCCCCGCGA |
NGG |
2 |
0.5625 |
Tier III |
| 9 |
NC_000070.6 |
4 |
124894124 |
+ |
1700125G02Rik |
NNNACAAGGTGTCCCCAAGG |
NGG |
2 |
0.5026 |
Tier III |
| 10 |
NC_000070.6 |
4 |
114780331 |
- |
Gm33655 |
NNNACAAGCTGTCCCCGCGG |
NCG |
2 |
0.0577 |
Tier III |
Other clones with same target sequence:
(none)
