Construct: sgRNA BRDN0001144830

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGACACGAAGCAGGAAACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK2 (23604)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76022

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 63926050 - DAPK2 NNNCACGAAGCAGGAAACAC NGG 0 1.0 Tier I
2 NC_000005.10 5 127229652 + LOC105379198 NNNCAGGAAGCAGGAAACCC NGG 2 0.1034 Tier I
3 NC_000012.12 12 21356703 + IAPP NNNCATGAAGCAGAAAACAC NGG 2 0.6964 Tier II
4 NC_000012.12 12 21356703 + SLCO1A2 NNNCATGAAGCAGAAAACAC NGG 2 0.6964 Tier II
5 NC_000010.11 10 127302142 - DOCK1 NNNCCAGAAGCAGGAAACAC NGG 2 0.4643 Tier II
6 NC_000009.12 9 132797093 + AK8 NNNCAGGAATCAGGAAACAC NGG 2 0.4063 Tier II
7 NC_000018.10 18 23420512 + TMEM241 NNNCAAGTAGCAGGAAACAC NGG 2 0.398 Tier II
8 NC_000023.11 X 29481693 - IL1RAPL1 NNNCACGAGGCAGGAAAAAC NGG 2 0.359 Tier II
9 NC_000017.11 17 72959915 - SLC39A11 NNNCACGAAGCAGGGAACTC NGG 2 0.35 Tier II
10 NC_000010.11 10 72138341 + ASCC1 NNNCACGAAGCATGAAATAC NGG 2 0.1929 Tier II
11 NC_000008.11 8 140989458 + PTK2 NNNCACAAAGCAGGAGACAC NGG 2 0.1923 Tier II
12 NC_000014.9 14 20504284 + RNASE10 NNNCACGAAGAAGGAAACCC NGG 2 0.1552 Tier II
13 NC_000005.10 5 127229652 + MEGF10 NNNCAGGAAGCAGGAAACCC NGG 2 0.1034 Tier II
14 NC_000005.10 5 54637924 + SNX18 NNNCACGCAGCAGGAAACCC NGG 2 0.0887 Tier II
15 NC_000017.11 17 29930364 - EFCAB5 NNNCACGAAGGAGGAAACCC NGG 2 0.0517 Tier II
16 NC_000017.11 17 28586236 + SPAG5 NNNCACTAAGCAGGAAACAG NGG 2 0.0336 Tier II
17 NC_000023.11 X 11326508 + ARHGAP6 NNNCACAAAGCAGGAAACAC NGT 2 0.0161 Tier II
18 NC_000008.11 8 18539962 - PSD3 NNNCAGGAAGCAGGAAACAC NGT 2 0.0081 Tier II
19 NC_000014.9 14 33154455 - NPAS3 NNNCACGAAGCAGGAGACAC NTG 2 0.0075 Tier II
20 NC_000006.12 6 39384298 + KIF6 NNNCACGAAGCAGGATACAG NGG 2 0.0 Tier II
21 NC_000010.11 10 133268359 - ADAM8 NNNCACGCAGCAGGACACAC NGG 2 0.0 Tier II
22 NC_000010.11 10 95903726 + ENTPD1-AS1 NNNCACGAAACAGGAAACAC NGG 1 0.9333 Tier III
23 NC_000009.12 9 33375034 + LOC124902142 NNNCCCAAAGCAGGAAACAC NGG 2 0.5 Tier III
24 NC_000003.12 3 54027211 + LOC105377095 NNNCTCGAAGCAGGAACCAC NGG 2 0.0642 Tier III
25 NC_000021.9 21 35987580 - LOC101928269 NNNCAAGAAGCAGGAAACAG NGG 2 0.0546 Tier III
26 NC_000002.12 2 9779763 + LOC100996549 NNNCAGGAAGCAGGAAACAC NGA 2 0.0347 Tier III
27 NC_000005.10 5 142036867 + MRPL11P2 NNNCACGAAGTAGGAAACAG NGG 2 0.0181 Tier III
28 NC_000007.14 7 156640096 + RNF32-DT NNNCACGAAGCAGGAAACAC NAC 2 0.0 Tier III
29 NC_000006.12 6 39384298 + LOC107986594 NNNCACGAAGCAGGATACAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 64555131 - Atp8b1 NNNTACGAAGCAAGAAACAC NGG 2 0.7385 Tier I
2 NC_000072.6 6 37953969 + Trim24 NNNCACAAAGAAGGAAACAC NGG 2 0.75 Tier II
3 NC_000083.6 17 27983168 - Anks1 NNNAACGAAGCAGAAAACAC NGG 2 0.6316 Tier II
4 NC_000076.6 10 119559578 - Grip1 NNNCACGAGGCAAGAAACAC NGG 2 0.6154 Tier II
5 NC_000073.6 7 134050421 + Adam12 NNNCATGAAGCAGGGAACAC NGG 2 0.6036 Tier II
6 NC_000067.6 1 151938458 + 1700025G04Rik NNNCACAATGCAGGAAACAC NGG 2 0.6 Tier II
7 NC_000080.6 14 61156352 - Sacs NNNCAAGAAGCAGGAAACTC NGG 2 0.5 Tier II
8 NC_000073.6 7 50747669 + Nell1 NNNCAGGAAGCAGAAAACAC NGG 2 0.375 Tier II
9 NC_000080.6 14 22500248 - Lrmda NNNCAGGATGCAGGAAACAC NGG 2 0.3 Tier II
10 NC_000085.6 19 43505123 + Got1 NNNCACCAAGCAGCAAACAC NGG 2 0.2946 Tier II
11 NC_000074.6 8 126448169 + Tarbp1 NNNCACGATGCAGCAAACAC NGG 2 0.2571 Tier II
12 NC_000067.6 1 152458661 + Colgalt2 NNNCAGGAAGCAGGAAACAA NGG 2 0.25 Tier II
13 NC_000071.6 5 36531300 + Tbc1d14 NNNCACAAAGCAGGAGACAC NGG 2 0.1923 Tier II
14 NC_000074.6 8 95687387 + Ndrg4 NNNCACCAAGCAGGCAACAC NGG 2 0.1875 Tier II
15 NC_000073.6 7 117416504 - Xylt1 NNNCACTAAGCATGAAACAC NGG 2 0.1714 Tier II
16 NC_000080.6 14 101866343 - Lmo7 NNNTACGAAGCAGGAAACCC NGG 2 0.1655 Tier II
17 NC_000073.6 7 76392744 - Agbl1 NNNCTCGAAGCAGCAAACAC NGG 2 0.1558 Tier II
18 NC_000077.6 11 35261011 - Slit3 NNNCACGAAGGAGGAAAAAC NGG 2 0.1346 Tier II
19 NC_000073.6 7 115644817 - Sox6 NNNCAGGAAGCAGGAAACAC NAG 2 0.1296 Tier II
20 NC_000077.6 11 33730413 - Kcnip1 NNNCAGGAAGGAGGAAACAC NGG 2 0.125 Tier II
21 NC_000084.6 18 65030135 - Nedd4l NNNCATGAAGCAGGAATCAC NGG 2 0.1238 Tier II
22 NC_000085.6 19 5038945 - B4gat1 NNNCACGAAGCAGGGACCAC NGG 2 0.1147 Tier II
23 NC_000072.6 6 113759394 - Atp2b2 NNNCACTAAGCAGGAACCAC NGG 2 0.1008 Tier II
24 NC_000071.6 5 32925467 - Depdc5 NNNCAGGAAGCAGGAAGCAC NGG 2 0.0882 Tier II
25 NC_000071.6 5 87815962 - Csn1s2b NNNCACTAAGCAGGAATCAC NGG 2 0.0762 Tier II
26 NC_000072.6 6 21955423 - Ing3 NNNCTCGAAGCAGGAAGCAC NGG 2 0.0642 Tier II
27 NC_000081.6 15 71885301 - Col22a1 NNNCAGGAAGCAGGAAACAG NGG 2 0.0294 Tier II
28 NC_000076.6 10 128937860 - Itga7 NNNCACGCAGCAGGAAACAC NGT 2 0.0069 Tier II
29 NC_000081.6 15 36952367 + Gm34590 NNNCATGAAGCAGAAAACAC NGG 2 0.6964 Tier III
30 NC_000073.6 7 134050421 + Gm39103 NNNCATGAAGCAGGGAACAC NGG 2 0.6036 Tier III
31 NC_000073.6 7 126626272 - Gm39087 NNNCACACAGCAGGAAACAC NGG 2 0.4286 Tier III
32 NC_000069.6 3 87508656 - Gm40085 NNNGACGAAGCAGAAAACAC NGG 2 0.375 Tier III
33 NC_000083.6 17 43794955 + Gm34773 NNNCATGAAGGAGGAAACAC NGG 2 0.2321 Tier III
34 NC_000076.6 10 128937860 - Gm46186 NNNCACGCAGCAGGAAACAC NGT 2 0.0069 Tier III
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Other clones with same target sequence:

(none)