Construct: sgRNA BRDN0001144834
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTAATGGTCACTTCGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD1 (5587)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77230
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
29638509 |
+ |
PRKD1 |
NNNTAATGGTCACTTCGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
19094057 |
+ |
DGCR2 |
NNNTAATGGTCACTTAACCA |
NGG |
2 |
0.9333 |
Tier II |
| 3 |
NC_000014.9 |
14 |
67527699 |
- |
GPHN |
NNNTAATGGACACTTCACCA |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000014.9 |
14 |
67527699 |
- |
TMEM229B |
NNNTAATGGACACTTCACCA |
NGG |
2 |
0.8 |
Tier II |
| 5 |
NC_000002.12 |
2 |
10347431 |
+ |
HPCAL1 |
NNNTAATGGTCACTTCGCCT |
NAG |
2 |
0.1556 |
Tier II |
| 6 |
NC_000011.10 |
11 |
19636019 |
- |
NAV2 |
NNNTAATGGTCACTTAGCCA |
NTG |
2 |
0.039 |
Tier II |
| 7 |
NC_000006.12 |
6 |
14512502 |
- |
LOC101928331 |
NNNTACTGGTCACTTTGCCA |
NGG |
2 |
0.1399 |
Tier III |
| 8 |
NC_000008.11 |
8 |
97305157 |
+ |
LOC101927066 |
NNNTAATGTTCACTTCTCCA |
NGG |
2 |
0.1333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
3337912 |
+ |
Pik3ip1 |
NNNTAATGGTCACTTCACCC |
NGG |
2 |
0.2121 |
Tier II |
Other clones with same target sequence:
(none)
