Construct: sgRNA BRDN0001144837
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGAGTATGCCTGTCCAGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TK2 (7084)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77297
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
66531403 |
+ |
TK2 |
NNNAGTATGCCTGTCCAGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
14549589 |
- |
GRIP2 |
NNNAGGATGTCTGTCCAGCA |
NGG |
2 |
0.5378 |
Tier II |
| 3 |
NC_000009.12 |
9 |
69240556 |
+ |
TJP2 |
NNNAATATTCCTGTCCAGCA |
NGG |
2 |
0.4622 |
Tier II |
| 4 |
NC_000003.12 |
3 |
126875301 |
+ |
CHCHD6 |
NNNTGTGTGCCTGTCCAGCA |
NGG |
2 |
0.4492 |
Tier II |
| 5 |
NC_000009.12 |
9 |
78316609 |
- |
PSAT1 |
NNNAGTATGCAGGTCCAGCA |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000008.11 |
8 |
41744865 |
+ |
ANK1 |
NNNGGTATTCCTGTCCAGCA |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000004.12 |
4 |
182771143 |
+ |
TENM3 |
NNNAGAATGCCTGCCCAGCA |
NGG |
2 |
0.2476 |
Tier II |
| 8 |
NC_000012.12 |
12 |
111308684 |
+ |
CUX2 |
NNNTGTATGCCTGTCCTGCA |
NGG |
2 |
0.0848 |
Tier II |
| 9 |
NC_000002.12 |
2 |
98356435 |
+ |
CNGA3 |
NNNAGTATGCCTGTCCATCA |
NGA |
2 |
0.0463 |
Tier II |
| 10 |
NC_000013.11 |
13 |
51036515 |
+ |
GUCY1B2 |
NNNAGAATGCTTGTCCAGCA |
NGG |
2 |
0.2667 |
Tier III |
| 11 |
NC_000016.10 |
16 |
11355844 |
+ |
LOC105371082 |
NNNAGTATCCCTGCCCAGCA |
NGG |
2 |
0.1538 |
Tier III |
| 12 |
NC_000001.11 |
1 |
160011367 |
- |
LOC124904436 |
NNNCTTATGCCTGTCCAGCA |
NGG |
2 |
0.1059 |
Tier III |
| 13 |
NC_000012.12 |
12 |
111308684 |
+ |
LOC105369983 |
NNNTGTATGCCTGTCCTGCA |
NGG |
2 |
0.0848 |
Tier III |
| 14 |
NC_000010.11 |
10 |
6925574 |
+ |
LOC105376387 |
NNNAGTATGCCTGTTCAGCA |
NGC |
2 |
0.0015 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
5346544 |
+ |
Klhl29 |
NNNAGTAAGCCTGTCCAGAA |
NGG |
2 |
0.3429 |
Tier II |
| 2 |
NC_000075.6 |
9 |
44832315 |
- |
Kmt2a |
NNNACTATGCCTCTCCAGCA |
NGG |
2 |
0.3308 |
Tier II |
| 3 |
NC_000069.6 |
3 |
148965241 |
- |
Adgrl2 |
NNNAGTGTGCCTGTCCAGAA |
NGG |
2 |
0.3025 |
Tier II |
| 4 |
NC_000084.6 |
18 |
23827972 |
+ |
Mapre2 |
NNNAGTGTGCTTGTCCAGCA |
NGG |
2 |
0.2172 |
Tier II |
| 5 |
NC_000078.6 |
12 |
29936960 |
+ |
Pxdn |
NNNAGTCTGCCTGTCCAGAA |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000077.6 |
11 |
107998795 |
+ |
Prkca |
NNNAGTATGCATGTCCAGGA |
NGG |
2 |
0.0938 |
Tier II |
| 7 |
NC_000078.6 |
12 |
29936960 |
+ |
Gm31939 |
NNNAGTCTGCCTGTCCAGAA |
NGG |
2 |
0.1875 |
Tier III |
| 8 |
NC_000067.6 |
1 |
71730168 |
- |
Gm30160 |
NNNAGTATGCCTGTCCAGGA |
NGA |
2 |
0.0087 |
Tier III |
| 9 |
NC_000067.6 |
1 |
71730168 |
- |
Gm39660 |
NNNAGTATGCCTGTCCAGGA |
NGA |
2 |
0.0087 |
Tier III |
Other clones with same target sequence:
(none)
