Construct: sgRNA BRDN0001144839
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGTTTGATGTGTGCCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MINK1 (50488)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76259
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
4887712 |
- |
MINK1 |
NNNGTTTGATGTGTGCCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
712218 |
+ |
OR4F16 |
NNNGCTTGATGTGTGCCTCA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000003.12 |
3 |
126471240 |
- |
ZXDC |
NNNGTTTGATGTGTGACTCT |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000019.10 |
19 |
1459851 |
- |
APC2 |
NNNGTTTGATAGGTGCCTCG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000002.12 |
2 |
73012189 |
- |
SFXN5 |
NNNGTTTGGTGTGTGCCTCT |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000019.10 |
19 |
58040769 |
+ |
ZSCAN1 |
NNNGTTTGCTGTGTGCCTCT |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000022.11 |
22 |
23300834 |
- |
BCR |
NNNGTTTTATGTGAGCCTCG |
NGG |
2 |
0.3869 |
Tier II |
| 8 |
NC_000023.11 |
X |
153784634 |
- |
SRPK3 |
NNNGTTTGCTGTGAGCCTCG |
NGG |
2 |
0.3537 |
Tier II |
| 9 |
NC_000005.10 |
5 |
151111174 |
- |
ANXA6 |
NNNGTTTGCTGTGTGCCTCC |
NGG |
2 |
0.2449 |
Tier II |
| 10 |
NC_000001.11 |
1 |
121259 |
+ |
LOC124900384 |
NNNGCTTGATGTGTGCCTCA |
NGG |
2 |
0.9375 |
Tier III |
| 11 |
NC_000002.12 |
2 |
73012189 |
- |
LOC107985897 |
NNNGTTTGGTGTGTGCCTCT |
NGG |
2 |
0.4667 |
Tier III |
| 12 |
NC_000011.10 |
11 |
107273694 |
+ |
LOC105369477 |
NNNGTTTGATGTGTGTCTAG |
NGG |
2 |
0.1319 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
70605127 |
- |
Mink1 |
NNNGTTTGATGTGTGCCTCC |
NGG |
1 |
0.4286 |
Tier I |
| 2 |
NC_000072.6 |
6 |
120934423 |
- |
Mical3 |
NNNGTTTGATGTGTGCCCCA |
NGG |
2 |
0.4018 |
Tier II |
| 3 |
NC_000076.6 |
10 |
110030060 |
- |
Nav3 |
NNNGTTTGATTTGTGCCTCA |
NGG |
2 |
0.3606 |
Tier II |
| 4 |
NC_000072.6 |
6 |
77484025 |
- |
Ctnna2 |
NNNGTTTAATGTGTGTCTCG |
NGG |
2 |
0.3077 |
Tier II |
| 5 |
NC_000071.6 |
5 |
147697083 |
- |
Flt1 |
NNNGTTTGGTGTGTGCCTAG |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000072.6 |
6 |
114467010 |
+ |
Hrh1 |
NNNGTTTGAAGTGTCCCTCG |
NGG |
2 |
0.2338 |
Tier II |
| 7 |
NC_000074.6 |
8 |
104347027 |
- |
Cmtm3 |
NNNGTTTGATGTGTGCTTCG |
NTG |
2 |
0.0182 |
Tier II |
| 8 |
NC_000073.6 |
7 |
67824114 |
+ |
Gm34350 |
NNNGTTTGGTGTCTGCCTCG |
NGG |
2 |
0.2807 |
Tier III |
Other clones with same target sequence:
(none)
