Construct: sgRNA BRDN0001144841
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATCAAGGGCTGACCACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TGFBR3 (7049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76292
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
91719348 |
+ |
TGFBR3 |
NNNTCAAGGGCTGACCACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
17763856 |
+ |
FAM184B |
NNNTCAAGGGCAGACCACCC |
NGG |
2 |
0.3429 |
Tier II |
| 3 |
NC_000005.10 |
5 |
135601126 |
+ |
SLC25A48 |
NNNTGAAGGGCTGACCACTG |
NGG |
2 |
0.2769 |
Tier II |
| 4 |
NC_000017.11 |
17 |
28971746 |
+ |
SEZ6 |
NNNTCAAGGGTTGACCACCT |
NGG |
2 |
0.2154 |
Tier II |
| 5 |
NC_000002.12 |
2 |
159636529 |
- |
BAZ2B |
NNNTCAAGTGCTGACCACCG |
NGA |
2 |
0.037 |
Tier II |
| 6 |
NC_000001.11 |
1 |
70279794 |
- |
ANKRD13C |
NNNTCAAGGGCTGATCACAG |
NGG |
2 |
0.0286 |
Tier II |
| 7 |
NC_000017.11 |
17 |
28971746 |
+ |
LOC105371716 |
NNNTCAAGGGTTGACCACCT |
NGG |
2 |
0.2154 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
59062416 |
- |
Sh3rf3 |
NNNTCAAGAGCTGACCACCT |
NGG |
2 |
0.45 |
Tier II |
| 2 |
NC_000083.6 |
17 |
25193560 |
- |
Unkl |
NNNGCAAGGGCTGACCACTG |
NGG |
2 |
0.2885 |
Tier II |
| 3 |
NC_000078.6 |
12 |
3871000 |
+ |
Dnmt3a |
NNNTCATGGGCTGAGCACCG |
NGG |
2 |
0.0219 |
Tier II |
| 4 |
NC_000081.6 |
15 |
79402865 |
+ |
Tmem184b |
NNNTCAAGGGCCGACCACCG |
NGC |
2 |
0.0211 |
Tier II |
Other clones with same target sequence:
(none)
