Construct: sgRNA BRDN0001144843
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAATACCTCCACTCACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K14 (9020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77736
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
45274151 |
- |
MAP3K14 |
NNNAATACCTCCACTCACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
8925516 |
+ |
C11orf16 |
NNNAATACCTCCACTCAGGC |
NGG |
2 |
0.0303 |
Tier I |
| 3 |
NC_000007.14 |
7 |
95957883 |
+ |
DYNC1I1 |
NNNAATACCACCACTCACGA |
NAG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000013.11 |
13 |
25605913 |
- |
ATP8A2 |
NNNATTACCTCCACTCAAGA |
NGG |
2 |
0.1958 |
Tier II |
| 5 |
NC_000016.10 |
16 |
3964974 |
+ |
ADCY9 |
NNNAACACCTCCACTCCCGA |
NGG |
2 |
0.1604 |
Tier II |
| 6 |
NC_000001.11 |
1 |
23906334 |
+ |
CNR2 |
NNNAATACCTCCACTCAGGA |
NTG |
2 |
0.0052 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
42959698 |
- |
Scml4 |
NNNAATACCACCACTCACTA |
NGG |
2 |
0.5714 |
Tier II |
| 2 |
NC_000080.6 |
14 |
72449479 |
- |
Gm9195 |
NNNAAAACCTCCACTCATGA |
NGG |
2 |
0.5571 |
Tier II |
| 3 |
NC_000073.6 |
7 |
46112345 |
+ |
Abcc8 |
NNNAATACCTCCACTCAAGG |
NGG |
2 |
0.4118 |
Tier II |
| 4 |
NC_000071.6 |
5 |
31096307 |
- |
Slc30a3 |
NNNAATACTTCCACTCCCGA |
NGG |
2 |
0.1544 |
Tier II |
| 5 |
NC_000077.6 |
11 |
121044093 |
+ |
Gm11775 |
NNNGATAGCTCCACTCACGA |
NGG |
2 |
0.4018 |
Tier III |
| 6 |
NC_000075.6 |
9 |
118905331 |
+ |
LOC102636563 |
NNNACTAACTCCACTCACGA |
NGG |
2 |
0.325 |
Tier III |
| 7 |
NC_000072.6 |
6 |
50503570 |
- |
Gm40372 |
NNNAATCCCTCCACTCAGGA |
NGG |
2 |
0.0583 |
Tier III |
Other clones with same target sequence:
(none)
