Construct: sgRNA BRDN0001144856
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTATTCACAAAAGAGCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MPP6 (51678)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000007.14 | 7 | 24649751 | + | PALS2 | NNNTATTCACAAAAGAGCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000016.10 | 16 | 1451717 | + | CLCN7 | NNNTCTGCACAAAAGAGCTG | NGG | 2 | 0.2941 | Tier I |
3 | NC_000002.12 | 2 | 232984809 | + | NGEF | NNNTATTTACAAAAAAGCTG | NGG | 2 | 0.8235 | Tier II |
4 | NC_000004.12 | 4 | 86102308 | - | MAPK10 | NNNTATTCACAAAAGAGTTG | NGG | 1 | 0.6429 | Tier II |
5 | NC_000008.11 | 8 | 86052616 | - | PSKH2 | NNNTATCCACAAAAGAACTG | NGG | 2 | 0.6417 | Tier II |
6 | NC_000015.10 | 15 | 72582552 | + | ARIH1 | NNNTATTCACAAATGAGCTA | NGG | 2 | 0.5 | Tier II |
7 | NC_000014.9 | 14 | 29748581 | - | PRKD1 | NNNTATGCACAGAAGAGCTG | NGG | 2 | 0.4248 | Tier II |
8 | NC_000002.12 | 2 | 153662991 | - | GALNT13 | NNNCATTCACAAAAGAGTTG | NGG | 2 | 0.416 | Tier II |
9 | NC_000002.12 | 2 | 108935729 | + | RANBP2 | NNNTATTCAACAAAGAGCTG | NGG | 2 | 0.3467 | Tier II |
10 | NC_000002.12 | 2 | 108935729 | + | EDAR | NNNTATTCAACAAAGAGCTG | NGG | 2 | 0.3467 | Tier II |
11 | NC_000003.12 | 3 | 169162120 | - | MECOM | NNNTATCCACCAAAGAGCTG | NGG | 2 | 0.275 | Tier II |
12 | NC_000011.10 | 11 | 99802789 | + | CNTN5 | NNNTCTTCACAAAAGAGATG | NGG | 2 | 0.2692 | Tier II |
13 | NC_000020.11 | 20 | 44967397 | - | STK4 | NNNTATTCACAAAACAACTG | NGG | 2 | 0.2545 | Tier II |
14 | NC_000007.14 | 7 | 87831320 | + | RUNDC3B | NNNTATTGAGAAAAGAGCTG | NGG | 2 | 0.25 | Tier II |
15 | NC_000015.10 | 15 | 76175177 | - | TMEM266 | NNNTCTTCACAAAGGAGCTG | NGG | 2 | 0.2333 | Tier II |
16 | NC_000001.11 | 1 | 49423899 | - | AGBL4 | NNNTATTCACTAATGAGCTG | NGG | 2 | 0.1641 | Tier II |
17 | NC_000010.11 | 10 | 115938533 | + | ATRNL1 | NNNTATTTACAAAATAGCTG | NGG | 2 | 0.125 | Tier II |
18 | NC_000014.9 | 14 | 64475579 | - | ZBTB25 | NNNTATTCACAAAAGGGTTG | NGG | 2 | 0.1236 | Tier II |
19 | NC_000015.10 | 15 | 47390710 | - | SEMA6D | NNNTCTTCACAAAAGACCTG | NGG | 2 | 0.1176 | Tier II |
20 | NC_000023.11 | X | 21987943 | - | SMS | NNNTATTCACAAATGGGCTG | NGG | 2 | 0.1026 | Tier II |
21 | NC_000003.12 | 3 | 29476627 | + | RBMS3 | NNNTATTGACAAAATAGCTG | NGG | 2 | 0.0918 | Tier II |
22 | NC_000020.11 | 20 | 32768752 | - | DNMT3B | NNNTTTTCACAAAAGAGCGG | NGG | 2 | 0.0909 | Tier II |
23 | NC_000019.10 | 19 | 11775247 | - | ZNF441 | NNNTTTTCACAAAAGACCTG | NGG | 2 | 0.0856 | Tier II |
24 | NC_000023.11 | X | 1379480 | + | IL3RA | NNNTATTCACAAAAGACCAG | NGG | 2 | 0.0672 | Tier II |
25 | NC_000024.10 | Y | 1379480 | + | IL3RA | NNNTATTCACAAAAGACCAG | NGG | 2 | 0.0672 | Tier II |
26 | NC_000023.11 | X | 48601663 | - | WDR13 | NNNTATTCACAATAGGGCTG | NGG | 2 | 0.0577 | Tier II |
27 | NC_000009.12 | 9 | 87666114 | - | DAPK1 | NNNTATTCACAGAAGAGCTG | NGA | 2 | 0.0502 | Tier II |
28 | NC_000003.12 | 3 | 4317662 | + | SUMF1 | NNNTATTCACAAAATAGCAG | NGG | 2 | 0.0408 | Tier II |
29 | NC_000010.11 | 10 | 67425353 | + | CTNNA3 | NNNTATACACAAAAGAGCTG | NTG | 2 | 0.0341 | Tier II |
30 | NC_000007.14 | 7 | 146484546 | + | CNTNAP2 | NNNTATTCACAAAAGAGTTG | NTG | 2 | 0.025 | Tier II |
31 | NC_000019.10 | 19 | 14162214 | - | ADGRL1 | NNNTATCCACAAAAGAGCTG | NGC | 2 | 0.0153 | Tier II |
32 | NC_000003.12 | 3 | 120636666 | + | HGD | NNNTATTCACAAAATAGCTG | NTG | 2 | 0.0056 | Tier II |
33 | NC_000016.10 | 16 | 2852831 | + | PRSS22 | NNNTATACACAAAAGCGCTG | NGG | 2 | 0.0 | Tier II |
34 | NC_000011.10 | 11 | 88880429 | - | GRM5 | NNNTATTCACAAAAGAGCTG | NCT | 2 | 0.0 | Tier II |
35 | NC_000008.11 | 8 | 122427709 | - | SMILR | NNNTATTTACAAAAGAGCTA | NGG | 2 | 0.8203 | Tier III |
36 | NT_187383.1 | 16 | 1789519 | - | LOC102724066 | NNNTATTCATAGAAGAGCTG | NGG | 2 | 0.6797 | Tier III |
37 | NC_000001.11 | 1 | 243962663 | - | LINC02774 | NNNCATTCAAAAAAGAGCTG | NGG | 2 | 0.5608 | Tier III |
38 | NC_000001.11 | 1 | 243962663 | - | LOC105373258 | NNNCATTCAAAAAAGAGCTG | NGG | 2 | 0.5608 | Tier III |
39 | NC_000010.11 | 10 | 112339110 | + | GUCY2GP | NNNTCTTGACAAAAGAGCTG | NGG | 2 | 0.3214 | Tier III |
40 | NC_000011.10 | 11 | 30850263 | - | LOC107984419 | NNNTATTAAGAAAAGAGCTG | NGG | 2 | 0.2528 | Tier III |
41 | NC_000015.10 | 15 | 76175177 | - | LOC101929439 | NNNTCTTCACAAAGGAGCTG | NGG | 2 | 0.2333 | Tier III |
42 | NC_000004.12 | 4 | 19503016 | + | LOC105374511 | NNNTATTCACAACAAAGCTG | NGG | 2 | 0.1981 | Tier III |
43 | NC_000001.11 | 1 | 49423899 | - | AGBL4-IT1 | NNNTATTCACTAATGAGCTG | NGG | 2 | 0.1641 | Tier III |
44 | NC_000003.12 | 3 | 151836026 | - | AADACL2-AS1 | NNNTATTAACAACAGAGCTG | NGG | 2 | 0.1368 | Tier III |
45 | NC_000001.11 | 1 | 244256021 | + | LOC105373262 | NNNTATTTACAAAAGAGGTG | NGG | 2 | 0.1167 | Tier III |
46 | NC_000004.12 | 4 | 14223021 | + | LOC124900670 | NNNTATTCACAATACAGCTG | NGG | 2 | 0.0818 | Tier III |
47 | NC_000001.11 | 1 | 84789872 | - | LOC102724892 | NNNTATACACAAAAGAGCTG | NGA | 2 | 0.0608 | Tier III |
48 | NC_000019.10 | 19 | 14162214 | - | ADGRL1-AS1 | NNNTATCCACAAAAGAGCTG | NGC | 2 | 0.0153 | Tier III |
49 | NC_000008.11 | 8 | 72123946 | - | LOC124901962 | NNNTATTCAGAAAAGTGCTG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000072.6 | 6 | 50162722 | + | Mpp6 | NNNTATTCACAAAAAAGCTG | NAG | 2 | 0.244 | Tier I |
2 | NC_000084.6 | 18 | 70469625 | + | 4930503L19Rik | NNNTATACACAAACGAGCTG | NGG | 2 | 0.1875 | Tier I |
3 | NC_000086.7 | X | 152652979 | - | Shroom2 | NNNTGTTCACAAAAGAGCTG | NGG | 1 | 0.72 | Tier II |
4 | NC_000083.6 | 17 | 52786250 | + | Kcnh8 | NNNTATTCACAAGAAAGCTG | NGG | 2 | 0.6138 | Tier II |
5 | NC_000085.6 | 19 | 34211224 | - | Stambpl1 | NNNCACTCACAAAAGAGCTG | NGG | 2 | 0.5882 | Tier II |
6 | NC_000069.6 | 3 | 65823712 | - | Lekr1 | NNNTATTGGCAAAAGAGCTG | NGG | 2 | 0.4286 | Tier II |
7 | NC_000069.6 | 3 | 121215497 | - | Tlcd4 | NNNTATTCAGAAAAGAACTG | NGG | 2 | 0.363 | Tier II |
8 | NC_000073.6 | 7 | 105785483 | + | Dchs1 | NNNTCTTCACAAATGAGCTG | NGG | 2 | 0.2667 | Tier II |
9 | NC_000079.6 | 13 | 77134676 | + | Slf1 | NNNTTTTCACAAAAGAGCTT | NGG | 2 | 0.2545 | Tier II |
10 | NC_000074.6 | 8 | 28879800 | - | Unc5d | NNNTATTCACAAAAGAACTG | NAG | 2 | 0.242 | Tier II |
11 | NC_000077.6 | 11 | 100430044 | - | Nt5c3b | NNNTTTTCACAAGAGAGCTG | NGG | 2 | 0.2372 | Tier II |
12 | NC_000076.6 | 10 | 90890767 | + | Anks1b | NNNTATACACAAAAGACCTG | NGG | 2 | 0.2059 | Tier II |
13 | NC_000077.6 | 11 | 95304443 | - | Kat7 | NNNTATTCAAAACAGAGCTG | NGG | 2 | 0.1825 | Tier II |
14 | NC_000067.6 | 1 | 140473340 | + | Kcnt2 | NNNTATTCACAAAAGGACTG | NGG | 2 | 0.1795 | Tier II |
15 | NC_000079.6 | 13 | 15631889 | - | Gli3 | NNNCATTCACAAAACAGCTG | NGG | 2 | 0.1765 | Tier II |
16 | NC_000080.6 | 14 | 28311478 | + | Erc2 | NNNTATTCACTAATGAGCTG | NGG | 2 | 0.1641 | Tier II |
17 | NC_000077.6 | 11 | 62197035 | - | Specc1 | NNNTATGCACACAAGAGCTG | NGG | 2 | 0.1548 | Tier II |
18 | NC_000067.6 | 1 | 107443475 | - | Serpinb7 | NNNCATTCACAACAGAGCTG | NGG | 2 | 0.1362 | Tier II |
19 | NC_000078.6 | 12 | 31699701 | - | Cog5 | NNNTATTCACAAAATAACTG | NGG | 2 | 0.1333 | Tier II |
20 | NC_000080.6 | 14 | 62277588 | + | Dleu7 | NNNTATTCATAAAAGAGCTG | NGA | 2 | 0.0654 | Tier II |
21 | NC_000081.6 | 15 | 53717983 | - | Samd12 | NNNTATTCACAAAACACCTG | NGG | 2 | 0.0642 | Tier II |
22 | NC_000086.7 | X | 52480302 | + | Gpc3 | NNNTATCCACAAAAGAGCTG | NTG | 2 | 0.0268 | Tier II |
23 | NC_000082.6 | 16 | 9896213 | + | Grin2a | NNNTATACACAAAAGAGCTG | NGC | 2 | 0.0194 | Tier II |
24 | NC_000084.6 | 18 | 15653322 | - | Chst9 | NNNTATTCACAAAAGATCTG | NGA | 2 | 0.0174 | Tier II |
25 | NC_000068.7 | 2 | 138684853 | - | Gm14061 | NNNTATTCACAGAAAAGCTG | NGG | 2 | 0.6797 | Tier III |
26 | NC_000072.6 | 6 | 4431372 | + | Gm33788 | NNNTATTCACAAAAGAACTC | NGG | 2 | 0.4 | Tier III |
27 | NC_000072.6 | 6 | 4431372 | + | Gm46948 | NNNTATTCACAAAAGAACTC | NGG | 2 | 0.4 | Tier III |
28 | NC_000069.6 | 3 | 21781787 | - | 7530428D23Rik | NNNTCTTCACAAAAGAGATG | NGG | 2 | 0.2692 | Tier III |
29 | NC_000068.7 | 2 | 75303816 | + | 2600014E21Rik | NNNTATTCACAAAAGAGATG | NAG | 2 | 0.1396 | Tier III |
30 | NC_000068.7 | 2 | 75303816 | + | Gm39857 | NNNTATTCACAAAAGAGATG | NAG | 2 | 0.1396 | Tier III |
31 | NC_000081.6 | 15 | 93695328 | - | Gm41387 | NNNTATTCATAAAATAGCTG | NGG | 2 | 0.1345 | Tier III |
32 | NC_000071.6 | 5 | 99596779 | + | A930011G23Rik | NNNTAATCACAAAAGAGCTG | NGA | 2 | 0.0602 | Tier III |