Construct: sgRNA BRDN0001144856

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGTATTCACAAAAGAGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPP6 (51678)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76774

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 24649751 + PALS2 NNNTATTCACAAAAGAGCTG NGG 0 1.0 Tier I
2 NC_000016.10 16 1451717 + CLCN7 NNNTCTGCACAAAAGAGCTG NGG 2 0.2941 Tier I
3 NC_000002.12 2 232984809 + NGEF NNNTATTTACAAAAAAGCTG NGG 2 0.8235 Tier II
4 NC_000004.12 4 86102308 - MAPK10 NNNTATTCACAAAAGAGTTG NGG 1 0.6429 Tier II
5 NC_000008.11 8 86052616 - PSKH2 NNNTATCCACAAAAGAACTG NGG 2 0.6417 Tier II
6 NC_000015.10 15 72582552 + ARIH1 NNNTATTCACAAATGAGCTA NGG 2 0.5 Tier II
7 NC_000014.9 14 29748581 - PRKD1 NNNTATGCACAGAAGAGCTG NGG 2 0.4248 Tier II
8 NC_000002.12 2 153662991 - GALNT13 NNNCATTCACAAAAGAGTTG NGG 2 0.416 Tier II
9 NC_000002.12 2 108935729 + RANBP2 NNNTATTCAACAAAGAGCTG NGG 2 0.3467 Tier II
10 NC_000002.12 2 108935729 + EDAR NNNTATTCAACAAAGAGCTG NGG 2 0.3467 Tier II
11 NC_000003.12 3 169162120 - MECOM NNNTATCCACCAAAGAGCTG NGG 2 0.275 Tier II
12 NC_000011.10 11 99802789 + CNTN5 NNNTCTTCACAAAAGAGATG NGG 2 0.2692 Tier II
13 NC_000020.11 20 44967397 - STK4 NNNTATTCACAAAACAACTG NGG 2 0.2545 Tier II
14 NC_000007.14 7 87831320 + RUNDC3B NNNTATTGAGAAAAGAGCTG NGG 2 0.25 Tier II
15 NC_000015.10 15 76175177 - TMEM266 NNNTCTTCACAAAGGAGCTG NGG 2 0.2333 Tier II
16 NC_000001.11 1 49423899 - AGBL4 NNNTATTCACTAATGAGCTG NGG 2 0.1641 Tier II
17 NC_000010.11 10 115938533 + ATRNL1 NNNTATTTACAAAATAGCTG NGG 2 0.125 Tier II
18 NC_000014.9 14 64475579 - ZBTB25 NNNTATTCACAAAAGGGTTG NGG 2 0.1236 Tier II
19 NC_000015.10 15 47390710 - SEMA6D NNNTCTTCACAAAAGACCTG NGG 2 0.1176 Tier II
20 NC_000023.11 X 21987943 - SMS NNNTATTCACAAATGGGCTG NGG 2 0.1026 Tier II
21 NC_000003.12 3 29476627 + RBMS3 NNNTATTGACAAAATAGCTG NGG 2 0.0918 Tier II
22 NC_000020.11 20 32768752 - DNMT3B NNNTTTTCACAAAAGAGCGG NGG 2 0.0909 Tier II
23 NC_000019.10 19 11775247 - ZNF441 NNNTTTTCACAAAAGACCTG NGG 2 0.0856 Tier II
24 NC_000023.11 X 1379480 + IL3RA NNNTATTCACAAAAGACCAG NGG 2 0.0672 Tier II
25 NC_000024.10 Y 1379480 + IL3RA NNNTATTCACAAAAGACCAG NGG 2 0.0672 Tier II
26 NC_000023.11 X 48601663 - WDR13 NNNTATTCACAATAGGGCTG NGG 2 0.0577 Tier II
27 NC_000009.12 9 87666114 - DAPK1 NNNTATTCACAGAAGAGCTG NGA 2 0.0502 Tier II
28 NC_000003.12 3 4317662 + SUMF1 NNNTATTCACAAAATAGCAG NGG 2 0.0408 Tier II
29 NC_000010.11 10 67425353 + CTNNA3 NNNTATACACAAAAGAGCTG NTG 2 0.0341 Tier II
30 NC_000007.14 7 146484546 + CNTNAP2 NNNTATTCACAAAAGAGTTG NTG 2 0.025 Tier II
31 NC_000019.10 19 14162214 - ADGRL1 NNNTATCCACAAAAGAGCTG NGC 2 0.0153 Tier II
32 NC_000003.12 3 120636666 + HGD NNNTATTCACAAAATAGCTG NTG 2 0.0056 Tier II
33 NC_000016.10 16 2852831 + PRSS22 NNNTATACACAAAAGCGCTG NGG 2 0.0 Tier II
34 NC_000011.10 11 88880429 - GRM5 NNNTATTCACAAAAGAGCTG NCT 2 0.0 Tier II
35 NC_000008.11 8 122427709 - SMILR NNNTATTTACAAAAGAGCTA NGG 2 0.8203 Tier III
36 NT_187383.1 16 1789519 - LOC102724066 NNNTATTCATAGAAGAGCTG NGG 2 0.6797 Tier III
37 NC_000001.11 1 243962663 - LINC02774 NNNCATTCAAAAAAGAGCTG NGG 2 0.5608 Tier III
38 NC_000001.11 1 243962663 - LOC105373258 NNNCATTCAAAAAAGAGCTG NGG 2 0.5608 Tier III
39 NC_000010.11 10 112339110 + GUCY2GP NNNTCTTGACAAAAGAGCTG NGG 2 0.3214 Tier III
40 NC_000011.10 11 30850263 - LOC107984419 NNNTATTAAGAAAAGAGCTG NGG 2 0.2528 Tier III
41 NC_000015.10 15 76175177 - LOC101929439 NNNTCTTCACAAAGGAGCTG NGG 2 0.2333 Tier III
42 NC_000004.12 4 19503016 + LOC105374511 NNNTATTCACAACAAAGCTG NGG 2 0.1981 Tier III
43 NC_000001.11 1 49423899 - AGBL4-IT1 NNNTATTCACTAATGAGCTG NGG 2 0.1641 Tier III
44 NC_000003.12 3 151836026 - AADACL2-AS1 NNNTATTAACAACAGAGCTG NGG 2 0.1368 Tier III
45 NC_000001.11 1 244256021 + LOC105373262 NNNTATTTACAAAAGAGGTG NGG 2 0.1167 Tier III
46 NC_000004.12 4 14223021 + LOC124900670 NNNTATTCACAATACAGCTG NGG 2 0.0818 Tier III
47 NC_000001.11 1 84789872 - LOC102724892 NNNTATACACAAAAGAGCTG NGA 2 0.0608 Tier III
48 NC_000019.10 19 14162214 - ADGRL1-AS1 NNNTATCCACAAAAGAGCTG NGC 2 0.0153 Tier III
49 NC_000008.11 8 72123946 - LOC124901962 NNNTATTCAGAAAAGTGCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 50162722 + Mpp6 NNNTATTCACAAAAAAGCTG NAG 2 0.244 Tier I
2 NC_000084.6 18 70469625 + 4930503L19Rik NNNTATACACAAACGAGCTG NGG 2 0.1875 Tier I
3 NC_000086.7 X 152652979 - Shroom2 NNNTGTTCACAAAAGAGCTG NGG 1 0.72 Tier II
4 NC_000083.6 17 52786250 + Kcnh8 NNNTATTCACAAGAAAGCTG NGG 2 0.6138 Tier II
5 NC_000085.6 19 34211224 - Stambpl1 NNNCACTCACAAAAGAGCTG NGG 2 0.5882 Tier II
6 NC_000069.6 3 65823712 - Lekr1 NNNTATTGGCAAAAGAGCTG NGG 2 0.4286 Tier II
7 NC_000069.6 3 121215497 - Tlcd4 NNNTATTCAGAAAAGAACTG NGG 2 0.363 Tier II
8 NC_000073.6 7 105785483 + Dchs1 NNNTCTTCACAAATGAGCTG NGG 2 0.2667 Tier II
9 NC_000079.6 13 77134676 + Slf1 NNNTTTTCACAAAAGAGCTT NGG 2 0.2545 Tier II
10 NC_000074.6 8 28879800 - Unc5d NNNTATTCACAAAAGAACTG NAG 2 0.242 Tier II
11 NC_000077.6 11 100430044 - Nt5c3b NNNTTTTCACAAGAGAGCTG NGG 2 0.2372 Tier II
12 NC_000076.6 10 90890767 + Anks1b NNNTATACACAAAAGACCTG NGG 2 0.2059 Tier II
13 NC_000077.6 11 95304443 - Kat7 NNNTATTCAAAACAGAGCTG NGG 2 0.1825 Tier II
14 NC_000067.6 1 140473340 + Kcnt2 NNNTATTCACAAAAGGACTG NGG 2 0.1795 Tier II
15 NC_000079.6 13 15631889 - Gli3 NNNCATTCACAAAACAGCTG NGG 2 0.1765 Tier II
16 NC_000080.6 14 28311478 + Erc2 NNNTATTCACTAATGAGCTG NGG 2 0.1641 Tier II
17 NC_000077.6 11 62197035 - Specc1 NNNTATGCACACAAGAGCTG NGG 2 0.1548 Tier II
18 NC_000067.6 1 107443475 - Serpinb7 NNNCATTCACAACAGAGCTG NGG 2 0.1362 Tier II
19 NC_000078.6 12 31699701 - Cog5 NNNTATTCACAAAATAACTG NGG 2 0.1333 Tier II
20 NC_000080.6 14 62277588 + Dleu7 NNNTATTCATAAAAGAGCTG NGA 2 0.0654 Tier II
21 NC_000081.6 15 53717983 - Samd12 NNNTATTCACAAAACACCTG NGG 2 0.0642 Tier II
22 NC_000086.7 X 52480302 + Gpc3 NNNTATCCACAAAAGAGCTG NTG 2 0.0268 Tier II
23 NC_000082.6 16 9896213 + Grin2a NNNTATACACAAAAGAGCTG NGC 2 0.0194 Tier II
24 NC_000084.6 18 15653322 - Chst9 NNNTATTCACAAAAGATCTG NGA 2 0.0174 Tier II
25 NC_000068.7 2 138684853 - Gm14061 NNNTATTCACAGAAAAGCTG NGG 2 0.6797 Tier III
26 NC_000072.6 6 4431372 + Gm33788 NNNTATTCACAAAAGAACTC NGG 2 0.4 Tier III
27 NC_000072.6 6 4431372 + Gm46948 NNNTATTCACAAAAGAACTC NGG 2 0.4 Tier III
28 NC_000069.6 3 21781787 - 7530428D23Rik NNNTCTTCACAAAAGAGATG NGG 2 0.2692 Tier III
29 NC_000068.7 2 75303816 + 2600014E21Rik NNNTATTCACAAAAGAGATG NAG 2 0.1396 Tier III
30 NC_000068.7 2 75303816 + Gm39857 NNNTATTCACAAAAGAGATG NAG 2 0.1396 Tier III
31 NC_000081.6 15 93695328 - Gm41387 NNNTATTCATAAAATAGCTG NGG 2 0.1345 Tier III
32 NC_000071.6 5 99596779 + A930011G23Rik NNNTAATCACAAAAGAGCTG NGA 2 0.0602 Tier III
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Other clones with same target sequence:

(none)