Construct: sgRNA BRDN0001144857
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGCTTCGTCAGTGGCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABCC1 (4363)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76858
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
16045972 |
+ |
ABCC1 |
NNNGCTTCGTCAGTGGCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
49857101 |
+ |
PTOV1 |
NNNGCTTCGTCAGTGCCATC |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000015.10 |
15 |
57965458 |
- |
ALDH1A2 |
NNNGCTTGGTCAGTGGCATA |
NGG |
2 |
0.6027 |
Tier II |
| 4 |
NC_000011.10 |
11 |
20468904 |
- |
PRMT3 |
NNNGCTTCGTCTGTGGCATA |
NGG |
2 |
0.3125 |
Tier II |
| 5 |
NC_000007.14 |
7 |
74586183 |
+ |
GTF2IRD1 |
NNNGATTCCTCAGTGGCATG |
NGG |
2 |
0.3077 |
Tier II |
| 6 |
NC_000012.12 |
12 |
112730792 |
- |
RPH3A |
NNNCCTTCTTCAGTGGCATG |
NGG |
2 |
0.2824 |
Tier II |
| 7 |
NC_000002.12 |
2 |
238280446 |
- |
PER2 |
NNNGCTTCCTCTGTGGCATG |
NGG |
2 |
0.1795 |
Tier II |
| 8 |
NC_000009.12 |
9 |
132770354 |
+ |
AK8 |
NNNGCTTCCTCAGTGCCATG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000011.10 |
11 |
23520412 |
+ |
RPS2P38 |
NNNGCTTTGTCAGTGGCATC |
NGG |
2 |
0.375 |
Tier III |
| 10 |
NC_000013.11 |
13 |
44651286 |
+ |
LINC00407 |
NNNGCTGCCTCAGTGGCATG |
NGG |
2 |
0.3167 |
Tier III |
| 11 |
NC_000007.14 |
7 |
66541388 |
+ |
RABGEF1P1 |
NNNGATTCCTCAGTGGCATG |
NGG |
2 |
0.3077 |
Tier III |
| 12 |
NC_000007.14 |
7 |
66541388 |
+ |
LOC100420547 |
NNNGATTCCTCAGTGGCATG |
NGG |
2 |
0.3077 |
Tier III |
| 13 |
NC_000014.9 |
14 |
74378508 |
- |
RPS2P2 |
NNNGCTTCGGCAGTGGCATC |
NGG |
2 |
0.2143 |
Tier III |
| 14 |
NC_000019.10 |
19 |
49857101 |
+ |
PTOV1-AS2 |
NNNGCTTCGTCAGTGCCATC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
14411585 |
+ |
Abcc1 |
NNNGCTTCGTCAGTGGCATG |
NGC |
1 |
0.0222 |
Tier I |
| 2 |
NC_000071.6 |
5 |
140185798 |
+ |
Mad1l1 |
NNNGCTTGGTCAGTGGCATG |
NGG |
1 |
0.6429 |
Tier II |
| 3 |
NC_000073.6 |
7 |
134763641 |
+ |
Dock1 |
NNNGCTTCATCAGTGGCATT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000083.6 |
17 |
32151836 |
- |
Notch3 |
NNNGTTTCCTCAGTGGCATG |
NGG |
2 |
0.3427 |
Tier II |
| 5 |
NC_000077.6 |
11 |
4485805 |
+ |
Mtmr3 |
NNNCCTTGGTCAGTGGCATG |
NGG |
2 |
0.3403 |
Tier II |
| 6 |
NC_000079.6 |
13 |
44897602 |
- |
Jarid2 |
NNNGCTTCGTCAGTGCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000085.6 |
19 |
54155747 |
+ |
Gm41865 |
NNNGCTTGGTCAGTGGCATG |
NGC |
2 |
0.0143 |
Tier III |
Other clones with same target sequence:
(none)
