Construct: sgRNA BRDN0001144863
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGTCTGCGACAAAACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKH (160851)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78043
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
42160082 |
+ |
DGKH |
NNNGTCTGCGACAAAACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
166945001 |
- |
SPOCK3 |
NNNGTCTGCAACAAGACATG |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000002.12 |
2 |
187217672 |
- |
CALCRL-AS1 |
NNNGTCTGTGACAAAACGTG |
NGG |
2 |
0.35 |
Tier III |
| 4 |
NC_000023.11 |
X |
136970512 |
+ |
TM9SF5P |
NNNGTCTGCCACACAACATG |
NGG |
2 |
0.0857 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
78624432 |
- |
Dgkh |
NNNGTCTGTGACAAAACCTG |
NGG |
2 |
0.1667 |
Tier I |
| 2 |
NC_000067.6 |
1 |
139065140 |
+ |
Dennd1b |
NNNGTCTGAAACAAAACATG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000079.6 |
13 |
8335690 |
- |
Adarb2 |
NNNGTCTGTGACAAAACTTG |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000084.6 |
18 |
5685789 |
- |
Zeb1 |
NNNTTCTGCAACAAAACATG |
NGG |
2 |
0.3394 |
Tier II |
| 5 |
NC_000067.6 |
1 |
57966220 |
+ |
Kctd18 |
NNNGTCAGCGACAACACATG |
NGG |
2 |
0.2386 |
Tier II |
| 6 |
NC_000075.6 |
9 |
65826030 |
+ |
Zfp609 |
NNNGTCTGCCACAAAGCATG |
NGG |
2 |
0.0769 |
Tier II |
| 7 |
NC_000067.6 |
1 |
19159905 |
- |
Tfap2d |
NNNGTCTGCCACAAAACATG |
NGA |
2 |
0.0278 |
Tier II |
| 8 |
NC_000080.6 |
14 |
67172677 |
+ |
Gm30438 |
NNNGGCTGTGACAAAACATG |
NGG |
2 |
0.56 |
Tier III |
Other clones with same target sequence:
(none)
