Construct: sgRNA BRDN0001144864

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTATAAAGAAACCTTAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUCY2C (2984)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77563

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 14683182 - GUCY2C NNNATAAAGAAACCTTAACC NGG 0 1.0 Tier I
2 NC_000003.12 3 195297202 - ACAP2 NNNATAAAGAAACCCCAACC NGG 2 0.1818 Tier I
3 NC_000002.12 2 237358519 + COL6A3 NNNATAAAGAAATCTTTACC NGG 2 0.0933 Tier I
4 NC_000002.12 2 239966756 + NDUFA10 NNNACAAAGAAACCTGAACC NGG 2 0.3462 Tier II
5 NC_000015.10 15 78105157 - CIB2 NNNAAAAAAAAACCTTAACC NGG 2 0.3214 Tier II
6 NC_000004.12 4 153518955 + TMEM131L NNNATCAAGAGACCTTAACC NGG 2 0.2955 Tier II
7 NC_000007.14 7 69613987 - AUTS2 NNNATAAACAAACCTTAACA NGG 2 0.2692 Tier II
8 NC_000006.12 6 133464953 - EYA4 NNNATAAAGAAACCTTATTC NGG 2 0.2308 Tier II
9 NC_000002.12 2 72501925 - EXOC6B NNNATAAAAAAACATTAACC NGG 2 0.225 Tier II
10 NC_000022.11 22 23624816 - DRICH1 NNNATATAGAAACCTTAATC NGG 2 0.2019 Tier II
11 NC_000016.10 16 57896243 + CNGB1 NNNATACAGAAACCTTAACC NAG 2 0.1134 Tier II
12 NC_000011.10 11 32904839 - QSER1 NNNATGAAGAAACCTTAAGC NGG 2 0.0893 Tier II
13 NC_000008.11 8 6524429 + ANGPT2 NNNATAAAGAAACCTTGAAC NGG 2 0.0756 Tier II
14 NC_000008.11 8 6524429 + MCPH1 NNNATAAAGAAACCTTGAAC NGG 2 0.0756 Tier II
15 NC_000013.11 13 79327776 + RBM26 NNNATAAAGAAACCATAAGC NGG 2 0.0724 Tier II
16 NC_000023.11 X 97106035 + DIAPH2 NNNATAAAGATAGCTTAACC NGG 2 0.042 Tier II
17 NC_000009.12 9 105454685 - FSD1L NNNATAAAGAAGCCTTAACC NTG 2 0.0281 Tier II
18 NC_000015.10 15 52402316 - MYO5A NNNATAAAGAAAACTTAACC NGA 2 0.0267 Tier II
19 NC_000007.14 7 31744240 - PDE1C NNNATAAAGAAACCTTAATC NGT 2 0.0074 Tier II
20 NC_000005.10 5 20299909 - CDH18 NNNATAAAGATACCTTAACC NGC 2 0.0068 Tier II
21 NC_000012.12 12 62745361 - PPM1H NNNATAAAGAAAAGTTAACC NGG 2 0.0 Tier II
22 NC_000008.11 8 47475775 - SPIDR NNNATAAAGAAACCTTAACC NTT 2 0.0 Tier II
23 NC_000012.12 12 14683182 - GUCY2C-AS1 NNNATAAAGAAACCTTAACC NGG 0 1.0 Tier III
24 NC_000012.12 12 58998537 + LRIG3-DT NNNATAAAGAAAACTAAACC NGG 2 0.3497 Tier III
25 NC_000005.10 5 152911857 - LINC01470 NNNATAAAAAAACCTTAACA NGG 2 0.3214 Tier III
26 NC_000002.12 2 51492543 + NRXN1-DT NNNATAAAGAATCCTTAAAC NGG 2 0.1429 Tier III
27 NC_000020.11 20 41957823 - LOC101927182 NNNATAAAGAAACCTTTCCC NGG 2 0.0254 Tier III
28 NC_000002.12 2 84243049 - LOC107985905 NNNGTAAAGAAACCTTAACC NTG 2 0.0244 Tier III
29 NC_000002.12 2 67573768 - LINC02831 NNNATAAAGAAACCATAACC NTG 2 0.0226 Tier III
30 NC_000002.12 2 67573768 - LOC105374786 NNNATAAAGAAACCATAACC NTG 2 0.0226 Tier III
31 NC_000002.12 2 126615666 + LOC105373602 NNNATAAAGAAAACTTAACC NGT 2 0.0062 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 136767129 - Gucy2c NNNATAAGGAAACCCTAACC NGG 2 0.2 Tier I
2 NC_000080.6 14 13390521 + Synpr NNNATAAAGTAACCTAAACC NGG 2 0.8021 Tier II
3 NC_000083.6 17 71304509 + Emilin2 NNNATAAATAAATCTTAACC NGG 2 0.3733 Tier II
4 NC_000074.6 8 33584073 + Tex15 NNNATTTAGAAACCTTAACC NGG 2 0.3125 Tier II
5 NC_000067.6 1 166206046 - Mael NNNATAAAAAAACCTTAAAC NGG 2 0.2755 Tier II
6 NC_000069.6 3 137427956 - Emcn NNNATTAAGAAACCTTAACT NGG 2 0.2143 Tier II
7 NC_000071.6 5 66980156 + Limch1 NNNATAAAGAAACCTTAGAC NGG 2 0.1714 Tier II
8 NC_000086.7 X 130080021 + Diaph2 NNNATAAAGAAAGCTTAACC NGG 1 0.1364 Tier II
9 NC_000075.6 9 123036077 + Tgm4 NNNAAAAAGAAACCTTAACC NAG 2 0.1296 Tier II
10 NC_000067.6 1 140251491 - Kcnt2 NNNATAATGAAACCTTAACC NAG 2 0.1111 Tier II
11 NC_000070.6 4 85527266 - Adamtsl1 NNNATAAAGAAACTTTAAGC NGG 2 0.0917 Tier II
12 NT_165789.2 X 103015 - Spry3 NNNATAAAGAAACCCTAACC NAG 2 0.0707 Tier II
13 NC_000067.6 1 40420034 - Il1rl1 NNNCTAAAGAAACCTTTACC NGG 2 0.0471 Tier II
14 NC_000075.6 9 16356971 - Fat3 NNNAGAAAGAAACCTTAACC NGA 2 0.0444 Tier II
15 NC_000086.7 X 134358336 - Cenpi NNNATAAAGAAATCTTAACG NGG 2 0.0412 Tier II
16 NC_000073.6 7 6174673 + Zfp444 NNNATAAAGAAACCCTAACC NCG 2 0.0292 Tier II
17 NC_000070.6 4 115637126 + Cyp4b1 NNNATAAAGAAACCTCAACC NTG 2 0.026 Tier II
18 NC_000069.6 3 13571308 - Ralyl NNNATAAAGAAACCTTAACA NGC 2 0.0111 Tier II
19 NC_000077.6 11 75323429 + Rpa1 NNNATAAAGAAACCTTAAAC NGT 2 0.0069 Tier II
20 NC_000071.6 5 20348779 - Magi2 NNNATAAATAAACGTTAACC NGG 2 0.0 Tier II
21 NC_000081.6 15 77276685 - Rbfox2 NNNATTAAGAAACGTTAACC NGG 2 0.0 Tier II
22 NC_000073.6 7 72746326 - Gm36865 NNNATACAGAAACCTTAACC NGG 1 0.4375 Tier III
23 NC_000073.6 7 38808520 - 1700010N08Rik NNNATAAAGAAACCTTAACT NAG 2 0.0778 Tier III
24 NC_000073.6 7 38944754 - Gm32110 NNNATAAAGAAACCTTAACT NAG 2 0.0778 Tier III
25 NC_000073.6 7 39299913 - Gm33382 NNNATAAAGAAACCTTAACT NAG 2 0.0778 Tier III
26 NC_000073.6 7 41073095 + 1700110I07Rik NNNATAAAGAAACCTTAACT NAG 2 0.0778 Tier III
27 NC_000074.6 8 76807820 + Gm10649 NNNATAAAGAAACCTTAACA NTG 2 0.0195 Tier III
28 NC_000074.6 8 76807820 + LOC115486409 NNNATAAAGAAACCTTAACA NTG 2 0.0195 Tier III
29 NC_000074.6 8 83764719 + Gm51542 NNNATAAATAAACCTTAACC NGT 2 0.0086 Tier III
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Other clones with same target sequence:

(none)