Construct: sgRNA BRDN0001144869
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGAGAACGACCTCAACCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ILK (3611)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75911
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
6604356 |
+ |
ILK |
NNNAGAACGACCTCAACCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
30695567 |
- |
IPO8 |
NNNAGAACGAGCTCAACCAG |
NTG |
2 |
0.0097 |
Tier I |
| 3 |
NC_000019.10 |
19 |
55257446 |
+ |
PPP6R1 |
NNNAGAAAGACCCCAACCAG |
NGG |
2 |
0.5132 |
Tier II |
| 4 |
NC_000018.10 |
18 |
44782105 |
+ |
SETBP1 |
NNNAGAACGACCTCAACCAG |
NAC |
2 |
0.0 |
Tier II |
| 5 |
NC_000016.10 |
16 |
20444488 |
- |
LOC105371119 |
NNNAGAAAGAGCTCAACCAG |
NGG |
2 |
0.1625 |
Tier III |
| 6 |
NC_000016.10 |
16 |
20583401 |
+ |
LOC105371120 |
NNNAGAAAGAGCTCAACCAG |
NGG |
2 |
0.1625 |
Tier III |
| 7 |
NC_000009.12 |
9 |
121562881 |
- |
LOC107987016 |
NNNAGAAAGACCTCAACCAG |
NGT |
2 |
0.0105 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
105737233 |
+ |
Ilk |
NNNAGAACGACCTCAATCAG |
NGG |
1 |
0.4667 |
Tier I |
| 2 |
NC_000073.6 |
7 |
105737233 |
+ |
Rrp8 |
NNNAGAACGACCTCAATCAG |
NGG |
1 |
0.4667 |
Tier I |
| 3 |
NC_000073.6 |
7 |
142016364 |
- |
Mob2 |
NNNAGATCGACCTCAACGAG |
NGG |
2 |
0.0583 |
Tier I |
| 4 |
NC_000079.6 |
13 |
38194912 |
+ |
Dsp |
NNNAGAACGACCTGAACCAG |
NGG |
1 |
0.0 |
Tier I |
| 5 |
NC_000082.6 |
16 |
52115301 |
- |
Cblb |
NNNAGAAAGAACTCAACCAG |
NGG |
2 |
0.4875 |
Tier II |
| 6 |
NC_000079.6 |
13 |
74020054 |
- |
Tppp |
NNNAGAATGACCTCAATCAG |
NGG |
2 |
0.4083 |
Tier II |
| 7 |
NC_000067.6 |
1 |
192367970 |
- |
Kcnh1 |
NNNAGAAGGACCTCAACCAC |
NGG |
2 |
0.2755 |
Tier II |
| 8 |
NC_000069.6 |
3 |
32611800 |
+ |
Gnb4 |
NNNAGAACGACTGCAACCAG |
NGG |
2 |
0.1405 |
Tier II |
| 9 |
NC_000074.6 |
8 |
57600292 |
+ |
Galnt7 |
NNNAGAACCACCTCAGCCAG |
NGG |
2 |
0.1036 |
Tier II |
| 10 |
NC_000083.6 |
17 |
45163686 |
- |
Gm4766 |
NNNTGAAAGACCTCAACCAG |
NGG |
2 |
0.4136 |
Tier III |
| 11 |
NC_000079.6 |
13 |
74020054 |
- |
Gm6263 |
NNNAGAATGACCTCAATCAG |
NGG |
2 |
0.4083 |
Tier III |
Other clones with same target sequence:
(none)
