Construct: sgRNA BRDN0001144871
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGAACACGTCCCCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK2 (156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75681
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
67279871 |
- |
GRK2 |
NNNGGAACACGTCCCCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
24940200 |
+ |
RUNX3 |
NNNGGGACACGTCCCCTCAG |
NGG |
2 |
0.5102 |
Tier II |
| 3 |
NC_000017.11 |
17 |
82559518 |
+ |
FOXK2 |
NNNGGAACACGTCCCCACGT |
NGG |
2 |
0.3733 |
Tier II |
| 4 |
NC_000022.11 |
22 |
20132402 |
- |
ZDHHC8 |
NNNGGCCCACGTCCCCTCGG |
NGG |
2 |
0.1989 |
Tier II |
| 5 |
NC_000005.10 |
5 |
64824704 |
- |
CWC27 |
NNNGGAACACTTCCCCTGGG |
NGG |
2 |
0.0513 |
Tier II |
| 6 |
NC_000001.11 |
1 |
24940200 |
+ |
RUNX3-AS1 |
NNNGGGACACGTCCCCTCAG |
NGG |
2 |
0.5102 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
4292200 |
+ |
Grk2 |
NNNGGAACACGTCCCCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
126396701 |
+ |
Nfatc2ip |
NNNGGACCACGTCCCCTCAG |
NGG |
2 |
0.3125 |
Tier I |
| 3 |
NC_000075.6 |
9 |
83590125 |
+ |
Sh3bgrl2 |
NNNGGAAGCCGTCCCCTCGG |
NGG |
2 |
0.3673 |
Tier II |
| 4 |
NC_000084.6 |
18 |
64608282 |
+ |
Atp8b1 |
NNNGGAACACGTCACCTCTG |
NGG |
2 |
0.2333 |
Tier II |
Other clones with same target sequence:
(none)
