Construct: sgRNA BRDN0001144875
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGATAAAGGTCCCACACGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK1 (3984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76046
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
74097127 |
- |
LIMK1 |
NNNTAAAGGTCCCACACGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
57365876 |
- |
RAE1 |
NNNTAAAAGTTCCACACGTG |
NGG |
2 |
0.3077 |
Tier II |
| 3 |
NC_000023.11 |
X |
96884017 |
- |
DIAPH2 |
NNNTAAAAGTCCCACACGAG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000023.11 |
X |
96884017 |
- |
RPA4 |
NNNTAAAAGTCCCACACGAG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000004.12 |
4 |
20996832 |
- |
KCNIP4 |
NNNTAAAGGGCCCACATGTG |
NGG |
2 |
0.2333 |
Tier II |
| 6 |
NC_000001.11 |
1 |
71017141 |
+ |
PTGER3 |
NNNTAAAGGACCCAGACGTG |
NGG |
2 |
0.0429 |
Tier II |
| 7 |
NC_000003.12 |
3 |
57971866 |
+ |
LOC105377104 |
NNNTCAAGGGCCCACACGTG |
NGG |
2 |
0.25 |
Tier III |
| 8 |
NC_000012.12 |
12 |
130317360 |
- |
LOC107984480 |
NNNTAAAGGTTCCACATGTG |
NGG |
2 |
0.1436 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
86084434 |
- |
Sh3d19 |
NNNTAAAGGTGCCACACCTG |
NGG |
2 |
0.119 |
Tier I |
| 2 |
NC_000075.6 |
9 |
106881242 |
- |
Rbm15b |
NNNTAAAAGTCCCACACATG |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000071.6 |
5 |
98447130 |
+ |
Cfap299 |
NNNTAAAGCTCCCACACGTG |
NAG |
2 |
0.1396 |
Tier II |
| 4 |
NC_000075.6 |
9 |
28594331 |
- |
Opcml |
NNNTAAAGGTCCCACACATG |
NGA |
2 |
0.0481 |
Tier II |
Other clones with same target sequence:
(none)
