Construct: sgRNA BRDN0001144876
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACCCCTCGAAGGACAACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK5 (203054)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77318
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
144390724 |
- |
ADCK5 |
NNNCCCTCGAAGGACAACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
80265001 |
- |
RNF213 |
NNNCCCTTGAAGGACAACAT |
NGG |
1 |
0.875 |
Tier II |
| 3 |
NC_000016.10 |
16 |
57901990 |
+ |
CNGB1 |
NNNCCCTGGAAGGACAACAT |
NAG |
2 |
0.1667 |
Tier II |
| 4 |
NC_000022.11 |
22 |
37209633 |
- |
SSTR3 |
NNNCCCTCGTAGGACATCAT |
NGG |
2 |
0.1176 |
Tier II |
| 5 |
NC_000007.14 |
7 |
102006344 |
+ |
CUX1 |
NNNCCCTCGAAGGACAACTC |
NGG |
2 |
0.049 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
35024254 |
- |
Vwa7 |
NNNCCTTCGAAGGACAACAT |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000083.6 |
17 |
85407131 |
- |
Camkmt |
NNNCCTTCGAAGGACAACAT |
NGG |
1 |
0.9286 |
Tier II |
| 3 |
NC_000077.6 |
11 |
21704976 |
- |
Wdpcp |
NNNCCCTCCAAGGTCAACAT |
NGG |
2 |
0.2872 |
Tier II |
| 4 |
NC_000067.6 |
1 |
188809233 |
+ |
Ush2a |
NNNCCCTGGAAGGAGAACAT |
NGG |
2 |
0.0321 |
Tier II |
| 5 |
NC_000081.6 |
15 |
79483518 |
+ |
Kcnj4 |
NNNCCCCCGAAGGACAACAT |
NTG |
2 |
0.0268 |
Tier II |
| 6 |
NC_000074.6 |
8 |
128322511 |
+ |
A930035F09Rik |
NNNCCCTCCAAGGACAACAT |
NGC |
2 |
0.012 |
Tier III |
Other clones with same target sequence:
(none)
