Construct: sgRNA BRDN0001144883
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCAACTGGACGCTCATCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMPR2 (659)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76391
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
202518868 |
- |
BMPR2 |
NNNAACTGGACGCTCATCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
76291970 |
- |
LRMDA |
NNNAACTGTATGCTCATCCA |
NGG |
2 |
0.1641 |
Tier II |
| 3 |
NC_000009.12 |
9 |
134837108 |
- |
COL5A1 |
NNNAACTGGCTGCTCATCCA |
NGG |
2 |
0.1026 |
Tier II |
| 4 |
NC_000004.12 |
4 |
7390501 |
+ |
SORCS2 |
NNNAACTGGGCGCTCATGCA |
NGG |
2 |
0.0741 |
Tier II |
| 5 |
NC_000017.11 |
17 |
17556317 |
+ |
PEMT |
NNNAACTGGATGCTCAGCCA |
NGG |
2 |
0.0362 |
Tier II |
| 6 |
NC_000005.10 |
5 |
41014645 |
- |
MROH2B |
NNNAACTGGAGGCTGATCCA |
NGG |
2 |
0.0125 |
Tier II |
| 7 |
NC_000006.12 |
6 |
150680383 |
- |
PLEKHG1 |
NNNAACTGGACCCTCTTCCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000012.12 |
12 |
95996593 |
- |
LOC102723340 |
NNNAGCTGGACTCTCATCCA |
NGG |
2 |
0.2769 |
Tier III |
| 9 |
NC_000005.10 |
5 |
5056685 |
+ |
LINC01020 |
NNNAACTGGGCTCTCATCCA |
NGG |
2 |
0.2137 |
Tier III |
| 10 |
NC_000010.11 |
10 |
76291970 |
- |
LOC124902462 |
NNNAACTGTATGCTCATCCA |
NGG |
2 |
0.1641 |
Tier III |
| 11 |
NC_000009.12 |
9 |
134837108 |
- |
LOC101448202 |
NNNAACTGGCTGCTCATCCA |
NGG |
2 |
0.1026 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
59845524 |
- |
Bmpr2 |
NNNAACTGGACGCTCATCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
85873500 |
+ |
Gtse1 |
NNNAACTTGACACTCATCCA |
NGG |
2 |
0.5833 |
Tier II |
| 3 |
NC_000080.6 |
14 |
27318225 |
+ |
Arhgef3 |
NNNAGCTGGAAGCTCATCCA |
NGG |
2 |
0.54 |
Tier II |
| 4 |
NC_000069.6 |
3 |
98645362 |
+ |
Hsd3b5 |
NNNCACTGGAAGCTCATCCA |
NGG |
2 |
0.2647 |
Tier II |
| 5 |
NC_000074.6 |
8 |
32328103 |
- |
Nrg1 |
NNNAACTGGAGGCTCATCCA |
NGG |
1 |
0.25 |
Tier II |
| 6 |
NC_000082.6 |
16 |
94191910 |
+ |
Hlcs |
NNNATCTGAACGCTCATCCA |
NGG |
2 |
0.2338 |
Tier II |
| 7 |
NC_000077.6 |
11 |
76275991 |
- |
Nxn |
NNNAACTGGACTCTCATCCC |
NGG |
2 |
0.0874 |
Tier II |
| 8 |
NC_000083.6 |
17 |
6125439 |
- |
Tulp4 |
NNNAACTGGTCGCTCATCCA |
NTG |
2 |
0.0344 |
Tier II |
| 9 |
NC_000067.6 |
1 |
86163281 |
- |
Armc9 |
NNNAACTGGAGGCTCATCCA |
NCG |
2 |
0.0268 |
Tier II |
| 10 |
NC_000070.6 |
4 |
152326922 |
+ |
Rpl22 |
NNNAACTGGACCCTCTTCCA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
