Construct: sgRNA BRDN0001144886
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACTGTACCAGACAAACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCQ (5588)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75553
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
6498405 |
+ |
PRKCQ |
NNNTGTACCAGACAAACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
123614334 |
+ |
MYLK |
NNNTGAACCAGACAACCTCG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000009.12 |
9 |
76167767 |
- |
PCSK5 |
NNNTTTACCAGACAAACTGG |
NGG |
2 |
0.0375 |
Tier II |
| 4 |
NC_000017.11 |
17 |
49209832 |
- |
GNGT2 |
NNNTGTACCAGACAACCTCC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000009.12 |
9 |
97781274 |
- |
PTCSC2 |
NNNTGGACCAGACAAACTCG |
NAG |
2 |
0.1481 |
Tier III |
| 6 |
NC_000003.12 |
3 |
123614334 |
+ |
MYLK-AS1 |
NNNTGAACCAGACAACCTCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
11245407 |
- |
Prkcq |
NNNTGTACCAGACAAATTCA |
NGG |
2 |
0.4375 |
Tier I |
| 2 |
NC_000073.6 |
7 |
110822587 |
+ |
Rnf141 |
NNNTCTAACAGACAAACTCG |
NGG |
2 |
0.5107 |
Tier II |
| 3 |
NC_000069.6 |
3 |
142290266 |
- |
Pdlim5 |
NNNTATACCAGACTAACTCG |
NGG |
2 |
0.4622 |
Tier II |
| 4 |
NC_000076.6 |
10 |
32509653 |
+ |
Nkain2 |
NNNTGTGCCAGACAAACTCC |
NGG |
2 |
0.3025 |
Tier II |
| 5 |
NC_000083.6 |
17 |
49009258 |
- |
Lrfn2 |
NNNTGTACCACACAAACACG |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000078.6 |
12 |
28558539 |
+ |
Allc |
NNNTGTACCAGACAGACTAG |
NGG |
2 |
0.2786 |
Tier II |
| 7 |
NC_000084.6 |
18 |
82927332 |
- |
Zfp516 |
NNNTGTACCAGCCAAACTGG |
NGG |
2 |
0.0329 |
Tier II |
| 8 |
NC_000084.6 |
18 |
36376402 |
+ |
Cystm1 |
NNNTGTACCAGAGAAACTCG |
NTG |
2 |
0.0053 |
Tier II |
| 9 |
NC_000083.6 |
17 |
22745664 |
- |
Gm5493 |
NNNTATACCAGACTAACTCG |
NGG |
2 |
0.4622 |
Tier III |
Other clones with same target sequence:
(none)
