Construct: sgRNA BRDN0001144888
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGGGAATTACCAGTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIPK1 (204851)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76995
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
113963517 |
- |
HIPK1 |
NNNGGGAATTACCAGTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
9986548 |
+ |
GAS7 |
NNNGGGAACTACCAGTACAG |
NGG |
1 |
0.9231 |
Tier II |
| 3 |
NC_000018.10 |
18 |
76479719 |
+ |
ZNF516 |
NNNGGGAAATACCAATACAG |
NGG |
2 |
0.8739 |
Tier II |
| 4 |
NC_000002.12 |
2 |
198113598 |
+ |
PLCL1 |
NNNAGGAAATACCAGTACAG |
NGG |
2 |
0.8357 |
Tier II |
| 5 |
NC_000005.10 |
5 |
172242713 |
+ |
UBTD2 |
NNNGGCAATTACCAGTACAG |
NGG |
1 |
0.6818 |
Tier II |
| 6 |
NC_000006.12 |
6 |
63305290 |
- |
LGSN |
NNNGGGAAATAGCAGTACAG |
NGG |
2 |
0.4127 |
Tier II |
| 7 |
NC_000006.12 |
6 |
165796585 |
+ |
PDE10A |
NNNGGGAATTACCACTACAG |
NGG |
1 |
0.2727 |
Tier II |
| 8 |
NC_000002.12 |
2 |
2046783 |
+ |
MYT1L |
NNNTGGAATTACTAGTACAG |
NGG |
2 |
0.2545 |
Tier II |
| 9 |
NC_000011.10 |
11 |
72705209 |
+ |
ARAP1 |
NNNGGGAGTTTCCAGTACAG |
NGG |
2 |
0.2256 |
Tier II |
| 10 |
NC_000017.11 |
17 |
27603309 |
- |
KSR1 |
NNNGGGAATTTCCAGCACAG |
NGG |
2 |
0.2051 |
Tier II |
| 11 |
NC_000016.10 |
16 |
877841 |
+ |
LMF1 |
NNNGGGAAGTACCAGTGCAG |
NGG |
2 |
0.1092 |
Tier II |
| 12 |
NC_000004.12 |
4 |
100035434 |
+ |
H2AZ1-DT |
NNNGGGAACTACCAGTAAAG |
NGG |
2 |
0.497 |
Tier III |
| 13 |
NC_000011.10 |
11 |
72705209 |
+ |
ARAP1-AS2 |
NNNGGGAGTTTCCAGTACAG |
NGG |
2 |
0.2256 |
Tier III |
| 14 |
NC_000002.12 |
2 |
170726080 |
+ |
ERICH2-DT |
NNNGGGAATTACCAGTACCA |
NGG |
2 |
0.194 |
Tier III |
| 15 |
NC_000014.9 |
14 |
56891853 |
+ |
OTX2-AS1 |
NNNGGGAATTACCAGTCCAG |
NGG |
1 |
0.1765 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
103753385 |
+ |
Hipk1 |
NNNGGGAATTACCAGTACAG |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000082.6 |
16 |
14401955 |
- |
Abcc1 |
NNNGGAAATTACCAGCACAG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000071.6 |
5 |
22117626 |
+ |
Reln |
NNNGGGAAATACCAGCACAG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000082.6 |
16 |
24525489 |
- |
Lpp |
NNNGGGAATTCCTAGTACAG |
NGG |
2 |
0.28 |
Tier II |
| 5 |
NC_000077.6 |
11 |
54110922 |
- |
P4ha2 |
NNNGGAAATTACCAGTACAG |
NAG |
2 |
0.2593 |
Tier II |
| 6 |
NC_000071.6 |
5 |
33261586 |
- |
Ctbp1 |
NNNTGGAAGTACCAGTACAG |
NGG |
2 |
0.2251 |
Tier II |
| 7 |
NC_000071.6 |
5 |
148084214 |
- |
Mtus2 |
NNNGGGAATCACCAGTACAG |
NAG |
2 |
0.1383 |
Tier II |
| 8 |
NC_000073.6 |
7 |
19418966 |
+ |
Ckm |
NNNGGGAATTACCAGTTCAA |
NGG |
2 |
0.125 |
Tier II |
| 9 |
NC_000070.6 |
4 |
96537312 |
- |
Cyp2j6 |
NNNGGGAATTACCAGAAGAG |
NGG |
2 |
0.1212 |
Tier II |
| 10 |
NC_000071.6 |
5 |
51921269 |
- |
Ppargc1a |
NNNGGGAATTACCTGTCCAG |
NGG |
2 |
0.0941 |
Tier II |
| 11 |
NC_000073.6 |
7 |
92613763 |
- |
Ankrd42 |
NNNGGGAATTACCAGTAGGG |
NGG |
2 |
0.05 |
Tier II |
| 12 |
NC_000068.7 |
2 |
78099402 |
+ |
4930440I19Rik |
NNNGCGCATTACCAGTACAG |
NGG |
2 |
0.3437 |
Tier III |
| 13 |
NC_000073.6 |
7 |
19418966 |
+ |
A930016O22Rik |
NNNGGGAATTACCAGTTCAA |
NGG |
2 |
0.125 |
Tier III |
Other clones with same target sequence:
(none)
