Construct: sgRNA BRDN0001144889
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCGACACAGCACTACAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CROT (54677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77708
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
87369404 |
- |
CROT |
NNNGACACAGCACTACAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
87369404 |
- |
ABCB4 |
NNNGACACAGCACTACAATG |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000005.10 |
5 |
34001716 |
+ |
AMACR |
NNNGAAACAGCACTACAATA |
NGG |
2 |
0.8705 |
Tier II |
| 4 |
NC_000016.10 |
16 |
18907404 |
- |
SMG1 |
NNNCACACTGCACTACAATG |
NGG |
2 |
0.3176 |
Tier II |
| 5 |
NC_000007.14 |
7 |
130420300 |
+ |
CEP41 |
NNNGCCACTGCACTACAATG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000003.12 |
3 |
7721711 |
+ |
GRM7 |
NNNGTCACATCACTACAATG |
NGG |
2 |
0.2955 |
Tier II |
| 7 |
NC_000015.10 |
15 |
66081850 |
- |
MEGF11 |
NNNCACACAGCACTACATTG |
NGG |
2 |
0.2647 |
Tier II |
| 8 |
NC_000002.12 |
2 |
17653220 |
- |
VSNL1 |
NNNGACACAGCCCTACAATT |
NGG |
2 |
0.1842 |
Tier II |
| 9 |
NC_000016.10 |
16 |
2266151 |
+ |
RNPS1 |
NNNGACACAGCACACCAATG |
NGG |
2 |
0.1688 |
Tier II |
| 10 |
NC_000016.10 |
16 |
70871741 |
+ |
HYDIN |
NNNGACACAGCTCTACAATC |
NGG |
2 |
0.1429 |
Tier II |
| 11 |
NC_000003.12 |
3 |
17008865 |
- |
PLCL2 |
NNNGCCACAGCACTCCAATG |
NGG |
2 |
0.1364 |
Tier II |
| 12 |
NC_000005.10 |
5 |
135570791 |
+ |
CXCL14 |
NNNAACACAGCAGTACAATG |
NGG |
2 |
0.1227 |
Tier II |
| 13 |
NC_000002.12 |
2 |
11362940 |
+ |
PPIAP60 |
NNNGACACAGCACTAGAATT |
NGG |
2 |
0.1077 |
Tier II |
| 14 |
NC_000009.12 |
9 |
35497229 |
+ |
RUSC2 |
NNNGACACAGCAGTACATTG |
NGG |
2 |
0.0682 |
Tier II |
| 15 |
NC_000012.12 |
12 |
99589037 |
- |
ANKS1B |
NNNGACACAGCACTACTTTG |
NGG |
2 |
0.0667 |
Tier II |
| 16 |
NC_000002.12 |
2 |
214235959 |
+ |
SPAG16 |
NNNGACACAGCAGTACAATG |
NGT |
2 |
0.0022 |
Tier II |
| 17 |
NC_000005.10 |
5 |
34001716 |
+ |
C1QTNF3-AMACR |
NNNGAAACAGCACTACAATA |
NGG |
2 |
0.8705 |
Tier III |
| 18 |
NC_000020.11 |
20 |
28582398 |
+ |
FRG1CP |
NNNGGCACAGCACTACAATC |
NGG |
2 |
0.3086 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
144794653 |
- |
Trrap |
NNNGACACAGCTCTACAAAG |
NGG |
2 |
0.0952 |
Tier I |
| 2 |
NC_000071.6 |
5 |
8983770 |
+ |
Crot |
NNNGACACAGCACGGCAATG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000082.6 |
16 |
60213576 |
- |
Epha6 |
NNNCACACTGCACTACAATG |
NGG |
2 |
0.3176 |
Tier II |
| 4 |
NC_000067.6 |
1 |
16562665 |
+ |
Ube2w |
NNNAACACAGCTCTACAATG |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000078.6 |
12 |
69348658 |
+ |
Nemf |
NNNGACACTGCACTACAATG |
NAG |
2 |
0.1556 |
Tier II |
| 6 |
NC_000071.6 |
5 |
122462897 |
+ |
Atp2a2 |
NNNGACACAGAACTTCAATG |
NGG |
2 |
0.15 |
Tier II |
| 7 |
NC_000069.6 |
3 |
108825284 |
+ |
Stxbp3 |
NNNGACCCAGCACCACAATG |
NGG |
2 |
0.125 |
Tier II |
| 8 |
NC_000070.6 |
4 |
151687389 |
- |
Camta1 |
NNNGACACAGCACTCTAATG |
NGG |
2 |
0.0839 |
Tier II |
| 9 |
NC_000073.6 |
7 |
37573178 |
- |
Zfp536 |
NNNGACACAGTACTACAATG |
NAG |
2 |
0.0798 |
Tier II |
| 10 |
NC_000073.6 |
7 |
31111832 |
- |
Hpn |
NNNGACACAGGACTACTATG |
NGG |
2 |
0.0333 |
Tier II |
| 11 |
NC_000068.7 |
2 |
104105707 |
+ |
Cd59a |
NNNGACACAGCAATACAATG |
NGA |
2 |
0.0267 |
Tier II |
| 12 |
NC_000069.6 |
3 |
37379520 |
+ |
Fgf2 |
NNNGACACAGCAGTACCATG |
NGG |
2 |
0.0241 |
Tier II |
| 13 |
NC_000067.6 |
1 |
24250487 |
- |
Col9a1 |
NNNGACACAGCAGGACAATG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000075.6 |
9 |
23144115 |
- |
Gm36743 |
NNNGACAGAGCACAACAATG |
NGG |
2 |
0.398 |
Tier III |
| 15 |
NC_000070.6 |
4 |
151687389 |
- |
Gm38442 |
NNNGACACAGCACTCTAATG |
NGG |
2 |
0.0839 |
Tier III |
| 16 |
NC_000068.7 |
2 |
52361935 |
+ |
Gm13521 |
NNNGACACAGCACTACAATG |
NCA |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
