Construct: sgRNA BRDN0001144892
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATGACTTCTCATCACGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR1 (90)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76759
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
157770440 |
+ |
ACVR1 |
NNNTGACTTCTCATCACGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
101305285 |
+ |
NALCN |
NNNTGCCTTCTCATCACTGG |
NGG |
2 |
0.303 |
Tier II |
| 3 |
NC_000003.12 |
3 |
56995941 |
- |
ARHGEF3 |
NNNTTACTTCTCATCACAGG |
NGG |
2 |
0.2077 |
Tier II |
| 4 |
NC_000012.12 |
12 |
18673744 |
+ |
PIK3C2G |
NNNTGACTTCTCATCAGGGG |
NAG |
2 |
0.0153 |
Tier II |
| 5 |
NC_000012.12 |
12 |
18673744 |
+ |
PLCZ1 |
NNNTGACTTCTCATCAGGGG |
NAG |
2 |
0.0153 |
Tier II |
| 6 |
NC_000016.10 |
16 |
6966715 |
- |
RBFOX1 |
NNNTGACTTCTCATCACAGG |
NGT |
2 |
0.0112 |
Tier II |
| 7 |
NC_000005.10 |
5 |
82813056 |
- |
LOC124901020 |
NNNTGACTTCTCATAACTGG |
NGG |
2 |
0.1481 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
58468586 |
+ |
Acvr1 |
NNNTGACTTCTCGTCTCGGG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000067.6 |
1 |
167007725 |
+ |
Fam78b |
NNNTGTCTTCTCATCACAGG |
NGG |
2 |
0.4945 |
Tier II |
| 3 |
NC_000081.6 |
15 |
79385564 |
- |
Tmem184b |
NNNTGACTTCTCGTCACTGG |
NGG |
2 |
0.4348 |
Tier II |
| 4 |
NC_000084.6 |
18 |
34518145 |
- |
Fam13b |
NNNTAACTTCTCATCAAGGG |
NGG |
2 |
0.4044 |
Tier II |
| 5 |
NC_000083.6 |
17 |
74675866 |
+ |
Birc6 |
NNNTTACTTCTCATCACTGG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000068.7 |
2 |
31332913 |
+ |
Hmcn2 |
NNNTGACTTCTCACCAAGGG |
NGG |
2 |
0.1333 |
Tier II |
| 7 |
NC_000071.6 |
5 |
117086075 |
- |
Gm42011 |
NNNTGACATCTCATCACGGG |
NTG |
2 |
0.0312 |
Tier III |
Other clones with same target sequence:
(none)
