Construct: sgRNA BRDN0001144893
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGTGACGTTAATGAACCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TIE1 (7075)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76687
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
43312026 |
+ |
TIE1 |
NNNTGACGTTAATGAACCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
120683686 |
- |
PRR16 |
NNNTGACATTACTGAACCTG |
NGG |
2 |
0.2632 |
Tier II |
| 3 |
NC_000006.12 |
6 |
133971241 |
- |
TBPL1 |
NNNTGACGTTAGTGAACCTG |
NAG |
2 |
0.1872 |
Tier II |
| 4 |
NC_000006.12 |
6 |
133971241 |
- |
LOC128092253 |
NNNTGACGTTAGTGAACCTG |
NAG |
2 |
0.1872 |
Tier II |
| 5 |
NC_000006.12 |
6 |
106186960 |
+ |
ATG5 |
NNNTGACGTTAATGAAACTG |
NAG |
2 |
0.121 |
Tier II |
| 6 |
NC_000012.12 |
12 |
14627855 |
- |
GUCY2C |
NNNTGAAGTTAATGAACGTG |
NGG |
2 |
0.1 |
Tier II |
| 7 |
NC_000001.11 |
1 |
65324969 |
+ |
DNAJC6 |
NNNTGAAGTTAATGAACCTG |
NTG |
2 |
0.0292 |
Tier II |
| 8 |
NC_000006.12 |
6 |
151301786 |
- |
AKAP12 |
NNNTGACGTTACTGAACCTG |
NGT |
2 |
0.0042 |
Tier II |
| 9 |
NC_000011.10 |
11 |
113770920 |
- |
ZW10 |
NNNTGACGTTAATGAGCCTG |
NGT |
2 |
0.0031 |
Tier II |
| 10 |
NC_000008.11 |
8 |
116456790 |
- |
LOC105375711 |
NNNTGATGATAATGAACCTG |
NGG |
2 |
0.7545 |
Tier III |
| 11 |
NC_000013.11 |
13 |
65287841 |
- |
STARP1 |
NNNTGACATTAATGAACCTG |
NAG |
2 |
0.2593 |
Tier III |
| 12 |
NC_000005.10 |
5 |
74063763 |
+ |
LINC02122 |
NNNTGACGTGAATGAATCTG |
NGG |
2 |
0.2333 |
Tier III |
| 13 |
NC_000010.11 |
10 |
122758953 |
+ |
DMBT1L1 |
NNNTGACGTTCATGAATCTG |
NGG |
2 |
0.1867 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
118480208 |
- |
Tie1 |
NNNTGACATTAATGAACCTG |
NAG |
2 |
0.2593 |
Tier I |
| 2 |
NC_000082.6 |
16 |
12096733 |
- |
Shisa9 |
NNNTGAAGCTAATGAACCTG |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000083.6 |
17 |
71070022 |
+ |
Myom1 |
NNNTGACGTTAACGAACTTG |
NGG |
2 |
0.5075 |
Tier II |
| 4 |
NC_000077.6 |
11 |
65102672 |
+ |
Arhgap44 |
NNNTGAAGTTAATGAACCCG |
NGG |
2 |
0.2069 |
Tier II |
| 5 |
NC_000074.6 |
8 |
108936506 |
+ |
Zfhx3 |
NNNTGATGTTAATGAGCCTG |
NGG |
2 |
0.1563 |
Tier II |
| 6 |
NC_000069.6 |
3 |
79568891 |
+ |
4930589L23Rik |
NNNAGACGTTAAAGAACCTG |
NGG |
2 |
0.3297 |
Tier III |
| 7 |
NC_000073.6 |
7 |
98419091 |
- |
Gm44507 |
NNNTGAGGTTAAGGAACCTG |
NGG |
2 |
0.1228 |
Tier III |
| 8 |
NC_000067.6 |
1 |
102983817 |
- |
Gm18728 |
NNNTGACATTAATGAACCTG |
NGA |
2 |
0.0694 |
Tier III |
Other clones with same target sequence:
(none)
